Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47128..47771 Replicon plasmid pEsST410_NW_3
Accession NZ_CP031233
Organism Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag DVH14_RS24245 Protein ID WP_001044768.1
Coordinates 47355..47771 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag DVH14_RS24240 Protein ID WP_001261287.1
Coordinates 47128..47358 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DVH14_RS24215 42288..43376 + 1089 WP_000952217.1 transcriptional repressor PifC -
DVH14_RS24220 43378..45603 + 2226 WP_000698737.1 phage T7 exclusion protein -
DVH14_RS24225 45653..46552 - 900 WP_000963206.1 nucleotide-binding protein -
DVH14_RS24230 46542..46832 - 291 WP_000111771.1 hypothetical protein -
DVH14_RS24240 47128..47358 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DVH14_RS24245 47355..47771 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
DVH14_RS24250 47933..49492 - 1560 WP_105075786.1 AAA family ATPase -
DVH14_RS24260 49774..50796 + 1023 WP_134889375.1 IS21 family transposase -
DVH14_RS24265 50793..51575 + 783 WP_134889376.1 IS21-like element IS100kyp family helper ATPase IstB -
DVH14_RS24270 51641..52030 - 390 Protein_55 AAA family ATPase -
DVH14_RS24275 52384..52641 + 258 WP_000343085.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / qepA1 iutA / iucD / iucC / iucB / iucA 1..98308 98308


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T108669 WP_001044768.1 NZ_CP031233:47355-47771 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T108669 NZ_CP031233:47355-47771 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT108669 WP_001261287.1 NZ_CP031233:47128-47358 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT108669 NZ_CP031233:47128-47358 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References