Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 16699..17224 Replicon plasmid pEsST410_NW_3
Accession NZ_CP031233
Organism Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DVH14_RS24065 Protein ID WP_001159871.1
Coordinates 16699..17004 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag DVH14_RS24070 Protein ID WP_000813630.1
Coordinates 17006..17224 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DVH14_RS24050 12661..13827 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
DVH14_RS24055 14415..15170 - 756 WP_000852146.1 replication initiation protein RepE -
DVH14_RS24060 15892..16698 - 807 WP_000016970.1 site-specific integrase -
DVH14_RS24065 16699..17004 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DVH14_RS24070 17006..17224 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DVH14_RS24080 17784..18014 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
DVH14_RS24085 18011..18427 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
DVH14_RS24090 18502..20067 + 1566 WP_001128474.1 AAA family ATPase -
DVH14_RS24095 20052..21074 + 1023 WP_000361402.1 helicase UvrD -
DVH14_RS24100 21328..22025 - 698 Protein_26 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sitABCD / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / qepA1 iutA / iucD / iucC / iucB / iucA 1..98308 98308


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T108667 WP_001159871.1 NZ_CP031233:c17004-16699 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T108667 NZ_CP031233:c17004-16699 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT108667 WP_000813630.1 NZ_CP031233:c17224-17006 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT108667 NZ_CP031233:c17224-17006 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References