Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 48400..49001 Replicon plasmid pEsST410_NW_2
Accession NZ_CP031232
Organism Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag DVH14_RS23900 Protein ID WP_001216045.1
Coordinates 48400..48780 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DVH14_RS23905 Protein ID WP_001190712.1
Coordinates 48780..49001 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DVH14_RS23875 43841..45325 - 1485 WP_000124150.1 terminase -
DVH14_RS23880 45325..46518 - 1194 WP_000219625.1 hypothetical protein -
DVH14_RS23885 46604..47056 - 453 WP_001326849.1 late promoter-activating protein -
DVH14_RS23890 47145..48188 - 1044 WP_023356283.1 DUF968 domain-containing protein -
DVH14_RS23895 48216..48395 - 180 WP_001339207.1 hypothetical protein -
DVH14_RS23900 48400..48780 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DVH14_RS23905 48780..49001 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DVH14_RS23910 49074..49463 - 390 WP_000506730.1 DNA repair protein -
DVH14_RS23915 49638..49853 + 216 Protein_60 DNA-binding protein -
DVH14_RS23920 49855..50229 + 375 Protein_61 integrase core domain-containing protein -
DVH14_RS23925 50800..52239 - 1440 WP_167551169.1 acyltransferase -
DVH14_RS23930 52296..52655 - 360 WP_134889374.1 acyltransferase -
DVH14_RS23935 52881..53627 + 747 Protein_64 IS1 family transposase -
DVH14_RS25750 53726..53905 - 180 Protein_65 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..54776 54776
- flank IS/Tn - - 49855..50229 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T108662 WP_001216045.1 NZ_CP031232:c48780-48400 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T108662 NZ_CP031232:c48780-48400 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT108662 WP_001190712.1 NZ_CP031232:c49001-48780 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT108662 NZ_CP031232:c49001-48780 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References