Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4040630..4041284 Replicon chromosome
Accession NZ_CP031231
Organism Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag DVH14_RS19785 Protein ID WP_000244777.1
Coordinates 4040630..4041037 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DVH14_RS19790 Protein ID WP_000354046.1
Coordinates 4041018..4041284 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DVH14_RS19765 4036587..4038320 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
DVH14_RS19770 4038326..4039036 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DVH14_RS19775 4039061..4039957 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DVH14_RS19780 4040069..4040590 + 522 WP_001055874.1 flavodoxin FldB -
DVH14_RS19785 4040630..4041037 - 408 WP_000244777.1 toxin CptA Toxin
DVH14_RS19790 4041018..4041284 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DVH14_RS19795 4041527..4042507 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
DVH14_RS19800 4042584..4043243 - 660 WP_000250274.1 hemolysin III family protein -
DVH14_RS19805 4043407..4043718 - 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
DVH14_RS19810 4043763..4045196 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
DVH14_RS19815 4045253..4045996 - 744 Protein_3805 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T108658 WP_000244777.1 NZ_CP031231:c4041037-4040630 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T108658 NZ_CP031231:c4041037-4040630 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT108658 WP_000354046.1 NZ_CP031231:c4041284-4041018 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT108658 NZ_CP031231:c4041284-4041018 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References