Detailed information of TA system TA108655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3599420..3600045
• Replicon: chromosome
• Accession: NZ_CP031231
• Organism: Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DVH14_RS17650
• Protein ID: WP_000911330.1
• Coordinates: 3599420..3599818 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: DVH14_RS17655
• Protein ID: WP_000450524.1
• Coordinates: 3599818..3600045 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVH14_RS17630	3595298..3595498	+	201	WP_000383836.1	YpfN family protein	-
DVH14_RS17635	3595608..3596306	-	699	WP_000679823.1	esterase	-
DVH14_RS17640	3596380..3598395	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
DVH14_RS17645	3598410..3599273	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
DVH14_RS17650	3599420..3599818	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DVH14_RS17655	3599818..3600045	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
DVH14_RS17660	3600199..3600912	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
DVH14_RS17665	3601125..3602159	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
DVH14_RS17670	3602176..3603054	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
DVH14_RS17675	3603200..3603772	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
DVH14_RS17680	3603772..3604242	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T108655 WP_000911330.1 NZ_CP031231:c3599818-3599420 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T108655 NZ_CP031231:c3599818-3599420 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT108655 WP_000450524.1 NZ_CP031231:c3600045-3599818 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT108655 NZ_CP031231:c3600045-3599818 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33