Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1368737..1369355 Replicon chromosome
Accession NZ_CP031231
Organism Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DVH14_RS06525 Protein ID WP_001291435.1
Coordinates 1368737..1368955 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DVH14_RS06530 Protein ID WP_000344800.1
Coordinates 1368981..1369355 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DVH14_RS06490 1364026..1364598 + 573 WP_000779831.1 YbaY family lipoprotein -
DVH14_RS06495 1364629..1364940 - 312 WP_000409911.1 MGMT family protein -
DVH14_RS06505 1365319..1365672 + 354 WP_000878140.1 DUF1428 domain-containing protein -
DVH14_RS06510 1365714..1367264 - 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DVH14_RS06515 1367428..1367898 - 471 WP_000136192.1 YlaC family protein -
DVH14_RS06520 1368014..1368565 - 552 WP_000102568.1 maltose O-acetyltransferase -
DVH14_RS06525 1368737..1368955 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DVH14_RS06530 1368981..1369355 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DVH14_RS06535 1369901..1373050 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
DVH14_RS06540 1373073..1374266 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T108645 WP_001291435.1 NZ_CP031231:c1368955-1368737 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T108645 NZ_CP031231:c1368955-1368737 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT108645 WP_000344800.1 NZ_CP031231:c1369355-1368981 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT108645 NZ_CP031231:c1369355-1368981 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References