Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE-Phd
Location 22769..23380 Replicon plasmid pPla107-2
Accession NZ_CP031228
Organism Pseudomonas amygdali pv. lachrymans str. M301315

Toxin (Protein)


Gene name PfiT Uniprot ID A0A3M3DN15
Locus tag PLA107_RS40185 Protein ID WP_005746121.1
Coordinates 22769..23119 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A3M5ISD9
Locus tag PLA107_RS40190 Protein ID WP_005746122.1
Coordinates 23132..23380 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PLA107_RS40150 18553..19173 - 621 WP_004642393.1 hypothetical protein -
PLA107_RS40155 19170..19436 - 267 WP_004642394.1 hypothetical protein -
PLA107_RS40160 19610..19858 + 249 Protein_28 hypothetical protein -
PLA107_RS40165 19944..20921 + 978 WP_007247761.1 IS5 family transposase -
PLA107_RS40170 21064..21738 + 675 Protein_30 IS3 family transposase -
PLA107_RS40775 21786..21902 - 117 Protein_31 prevent-host-death protein -
PLA107_RS40180 22112..22600 + 489 WP_005746120.1 hypothetical protein -
PLA107_RS40185 22769..23119 - 351 WP_005746121.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PLA107_RS40190 23132..23380 - 249 WP_005746122.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PLA107_RS40195 23630..24136 + 507 Protein_35 hypothetical protein -
PLA107_RS40200 24225..25202 + 978 WP_007247761.1 IS5 family transposase -
PLA107_RS40205 25791..27227 - 1437 WP_005746124.1 replication initiation protein -
PLA107_RS40210 27811..28065 - 255 WP_005746128.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40720 40720
- inside IScluster/Tn - - 5222..38323 33101


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12844.78 Da        Isoelectric Point: 4.5514

>T108635 WP_005746121.1 NZ_CP031228:c23119-22769 [Pseudomonas amygdali pv. lachrymans str. M301315]
MTTFALRFTEVAQQSLEDQVEHLAVTQGFSSAARRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSGGITGIVVLLVLGGKQSVEQALIRYCLLQLI

Download         Length: 351 bp

>T108635 NZ_CP031228:c23119-22769 [Pseudomonas amygdali pv. lachrymans str. M301315]
ATGACTACATTTGCACTGCGCTTTACCGAGGTTGCGCAGCAAAGCCTTGAAGATCAGGTTGAACACTTGGCTGTCACTCA
GGGGTTTTCATCAGCTGCTCGACGTATAGATTCCCTGATCGATGCCATTCAGGACAAGCTCCTGTCCACTCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTATTGCACTATCGTGAGTTGAACACTGACGGATACCGAATCTTTTAC
GAGGTGATGGATTCAGGTGGTATTACCGGCATCGTCGTATTGCTCGTATTAGGTGGTAAACAAAGTGTTGAACAGGCATT
GATCCGCTATTGCCTGCTTCAACTTATTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9405.72 Da        Isoelectric Point: 9.5019

>AT108635 WP_005746122.1 NZ_CP031228:c23380-23132 [Pseudomonas amygdali pv. lachrymans str. M301315]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFD
HK

Download         Length: 249 bp

>AT108635 NZ_CP031228:c23380-23132 [Pseudomonas amygdali pv. lachrymans str. M301315]
ATGCGAGTTGAAACAATAAGTTATCTGAAACGTAACGCAGCCGACCTGCCTCTCGATGAGCCACTGGTTGTCACGCAAAA
CGGTGTGCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGCCGCGATGAGTCCATCGCCTTAGTGAAGCTCCTGG
CAATCAGCTCTCGCGAATACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTGAT
CACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M3DN15

Antitoxin

Source ID Structure
AlphaFold DB A0A3M5ISD9

References