Detailed information of TA system TA108633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 787677..788254
• Replicon: plasmid pMPPla107
• Accession: NZ_CP031226
• Organism: Pseudomonas amygdali pv. lachrymans str. M301315

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A3M5I5K9
• Locus tag: PLA107_RS38705
• Protein ID: WP_005742667.1
• Coordinates: 787677..788012 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PLA107_RS38710
• Protein ID: WP_005742665.1
• Coordinates: 788009..788254 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLA107_RS38680	782772..783866	-	1095	WP_005742672.1	saccharopine dehydrogenase NADP-binding domain-containing protein	-
PLA107_RS38685	783868..784380	-	513	WP_147480938.1	hypothetical protein	-
PLA107_RS38690	784469..785572	-	1104	WP_054068257.1	hypothetical protein	-
PLA107_RS38695	785693..787141	-	1449	WP_147480939.1	sel1 repeat family protein	-
PLA107_RS38700	787275..787619	-	345	WP_005742668.1	hypothetical protein	-
PLA107_RS38705	787677..788012	-	336	WP_005742667.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PLA107_RS38710	788009..788254	-	246	WP_005742665.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PLA107_RS38715	788360..789913	-	1554	WP_005742664.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tufA	1..971889	971889

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-97)

Antitoxin

• SpoVT-AbrB domain (6-46)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12250.26 Da
• Isoelectric Point: 4.7960

>T108633 WP_005742667.1 NZ_CP031226:c788012-787677 [Pseudomonas amygdali pv. lachrymans str. M301315]
MIQQVPNSGDIAWLCIEPKPDDLHEERQPVLVLSPADYNLKTGLMLCCPMTTQIKGYPFEVEIDGEAPAAVLADQIRSLD
WKVFKAVVRGFVTQAELSSVRLRAASLIGKP

Nucleotide

• Length: 336 bp

>T108633 NZ_CP031226:c788012-787677 [Pseudomonas amygdali pv. lachrymans str. M301315]
ATGATTCAGCAGGTTCCTAACTCTGGTGATATAGCCTGGCTTTGCATTGAGCCCAAGCCTGATGATCTTCACGAGGAGCG
TCAGCCTGTTTTGGTGCTCAGTCCCGCTGACTACAACCTCAAGACTGGCCTGATGCTGTGTTGCCCAATGACGACACAGA
TCAAAGGATACCCTTTTGAGGTCGAAATCGATGGTGAAGCGCCAGCTGCAGTACTGGCTGACCAAATCAGAAGCCTGGAC
TGGAAGGTTTTCAAGGCAGTGGTGCGTGGCTTTGTTACACAGGCTGAATTGAGCTCTGTTCGATTGCGGGCAGCTTCCCT
GATTGGAAAGCCTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8969.31 Da
• Isoelectric Point: 6.0610

>AT108633 WP_005742665.1 NZ_CP031226:c788254-788009 [Pseudomonas amygdali pv. lachrymans str. M301315]
MQVIIKKWGNSPSVRIPASVMKDANLSVDDVVDVRAEHGRVIIEPVYPTEIDLHSLIAEITPENVHAQAHFGSLEIMGRA
R

Nucleotide

• Length: 246 bp

>AT108633 NZ_CP031226:c788254-788009 [Pseudomonas amygdali pv. lachrymans str. M301315]
ATGCAGGTCATTATCAAAAAATGGGGCAACAGCCCCTCGGTTCGAATTCCCGCCTCCGTAATGAAAGACGCCAATCTGTC
AGTAGATGATGTCGTGGATGTTCGTGCCGAGCACGGTCGAGTGATCATCGAGCCGGTCTATCCAACCGAGATAGATCTGC
ACTCTCTGATAGCTGAGATCACCCCTGAGAATGTTCATGCCCAAGCTCATTTCGGGTCTTTAGAAATCATGGGGAGAGCT
CGATGA

Structures

Toxin

• AlphaFold DB: A0A3M5I5K9