Detailed information of TA system TA108632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5738822..5739431
• Replicon: chromosome
• Accession: NZ_CP031225
• Organism: Pseudomonas amygdali pv. lachrymans str. M301315

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PLA107_RS32425
• Protein ID: WP_080164823.1
• Coordinates: 5738822..5739202 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3M6GEZ6
• Locus tag: PLA107_RS32430
• Protein ID: WP_005746660.1
• Coordinates: 5739192..5739431 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLA107_RS32395	5733976..5734506	+	531	WP_044299855.1	nucleotidyltransferase family protein	-
PLA107_RS32400	5734736..5735350	-	615	WP_005746667.1	histidine phosphatase family protein	-
PLA107_RS32405	5735454..5735795	-	342	WP_005746665.1	hypothetical protein	-
PLA107_RS32410	5735985..5736407	+	423	WP_005746664.1	DUF1493 family protein	-
PLA107_RS32415	5736959..5738044	+	1086	WP_005746663.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
PLA107_RS32420	5738178..5738915	+	738	WP_005746662.1	hypothetical protein	-
PLA107_RS32425	5738822..5739202	-	381	WP_080164823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PLA107_RS32430	5739192..5739431	-	240	WP_005746660.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
PLA107_RS32435	5739595..5740068	-	474	WP_005746659.1	GNAT family N-acetyltransferase	-
PLA107_RS32440	5740169..5740717	-	549	WP_005746658.1	GNAT family N-acetyltransferase	-
PLA107_RS32445	5740877..5741371	+	495	WP_004667254.1	hypothetical protein	-
PLA107_RS32450	5741405..5742679	-	1275	WP_004667253.1	OprD family porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-70)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13873.07 Da
• Isoelectric Point: 8.6903

>T108632 WP_080164823.1 NZ_CP031225:c5739202-5738822 [Pseudomonas amygdali pv. lachrymans str. M301315]
MTYNLELDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
RECPPATADESLTGVDQPSVAAFLAARAASIAASVSWVCSSPLYAS

Nucleotide

• Length: 381 bp

>T108632 NZ_CP031225:c5739202-5738822 [Pseudomonas amygdali pv. lachrymans str. M301315]
ATGACCTATAACCTCGAACTCGATGCGCGGGCTCTAAAGGAATGGCACAAATTAGGAGATACCGTGCGTCAGCAGCTCAA
GAAAAAGCTGGCTACGATTCTGGTTGCTCCGCGTGTTGAAGCCAATCGTCTGCATGCATTGCCTGACTGCTACAAAATCA
AACTGCGCAGTAGCGGCTATCGGCTGGTTTATCAGGTTATTGACCAAGAGGTTGTGGTCTTCGTGGTTGCGGTAGACAAG
CGTGAATGTCCGCCCGCAACGGCTGACGAGTCGCTGACTGGTGTTGATCAACCCTCAGTGGCGGCCTTTTTGGCCGCACG
TGCAGCGAGCATCGCCGCTTCCGTTTCCTGGGTGTGCAGCTCACCTTTGTACGCCAGTTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8999.40 Da
• Isoelectric Point: 4.6017

>AT108632 WP_005746660.1 NZ_CP031225:c5739431-5739192 [Pseudomonas amygdali pv. lachrymans str. M301315]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLATPQRVKISLDDL

Nucleotide

• Length: 240 bp

>AT108632 NZ_CP031225:c5739431-5739192 [Pseudomonas amygdali pv. lachrymans str. M301315]
ATGGCGTCTATCAACGTTCGTATCGACGACGATCTCAAAGCGCGTGCTTACCTCGAGCTGGAAAAGCTTGGCGTCACCCC
CTCGGAGTTGCTGCGCCAAACCCTGCAGTACGTAGCTGAGCGTGGTCAGCTACCCTTCAAGACGGTTCTGATGACAGAGG
AAGACGAAGCGCTGTTGGCAACTGTGCGTGAGCGCCTTGCAACTCCGCAGCGGGTCAAGATTTCACTGGATGACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A3M6GEZ6