Detailed information of TA system TA108625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4745558..4746139
• Replicon: chromosome
• Accession: NZ_CP031215
• Organism: Escherichia coli strain Es_ST80_L1_NDM_10_2017

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: V0SV58
• Locus tag: DV870_RS24395
• Protein ID: WP_000254750.1
• Coordinates: 4745804..4746139 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DV870_RS24390
• Protein ID: WP_114710177.1
• Coordinates: 4745558..4745791 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV870_RS24380	4741897..4743198	+	1302	WP_000046817.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
DV870_RS24385	4743246..4745480	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
DV870_RS24390	4745558..4745791	+	234	WP_114710177.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
DV870_RS24395	4745804..4746139	+	336	WP_000254750.1	endoribonuclease MazF	Toxin
DV870_RS24400	4746211..4747002	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
DV870_RS24405	4747230..4748867	+	1638	WP_021531536.1	CTP synthase (glutamine hydrolyzing)	-
DV870_RS24410	4748955..4750253	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12067.95 Da
• Isoelectric Point: 8.2618

>T108625 WP_000254750.1 NZ_CP031215:4745804-4746139 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T108625 NZ_CP031215:4745804-4746139 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8699.99 Da
• Isoelectric Point: 4.5515

>AT108625 WP_114710177.1 NZ_CP031215:4745558-4745791 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGAER

Nucleotide

• Length: 234 bp

>AT108625 NZ_CP031215:4745558-4745791 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGATCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGCCGAAAGATAA

Structures

Toxin

• AlphaFold DB: V0SV58