Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3084116..3084613 Replicon chromosome
Accession NZ_CP031215
Organism Escherichia coli strain Es_ST80_L1_NDM_10_2017

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag DV870_RS16025 Protein ID WP_001173343.1
Coordinates 3084116..3084289 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag DV870_RS16030 Protein ID WP_001312331.1
Coordinates 3084317..3084613 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV870_RS16015 3080786..3082243 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
DV870_RS16020 3082480..3083997 + 1518 WP_000003806.1 lysine--tRNA ligase -
DV870_RS16025 3084116..3084289 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
DV870_RS16030 3084317..3084613 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
DV870_RS16035 3084842..3085114 - 273 WP_000405647.1 N-acetyltransferase -
DV870_RS16040 3085126..3085356 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
DV870_RS16045 3085537..3087168 + 1632 WP_001216482.1 sensor histidine kinase -
DV870_RS16050 3087165..3087884 + 720 WP_001540474.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - iroB 3041987..3084613 42626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T108618 WP_001173343.1 NZ_CP031215:c3084289-3084116 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T108618 NZ_CP031215:c3084289-3084116 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT108618 WP_001312331.1 NZ_CP031215:c3084613-3084317 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT108618 NZ_CP031215:c3084613-3084317 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References