Detailed information of TA system TA108616

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 2821967..2822670
• Replicon: chromosome
• Accession: NZ_CP031215
• Organism: Escherichia coli strain Es_ST80_L1_NDM_10_2017

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A0L0H2I3
• Locus tag: DV870_RS14700
• Protein ID: WP_021530985.1
• Coordinates: 2821967..2822308 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: DV870_RS14705
• Protein ID: WP_021530984.1
• Coordinates: 2822329..2822670 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV870_RS14675	2817223..2817402	+	180	WP_001298063.1	hypothetical protein	-
DV870_RS14680	2817801..2819837	-	2037	WP_000417001.1	hypothetical protein	-
DV870_RS14690	2820709..2821716	-	1008	WP_004108748.1	restriction endonuclease	-
DV870_RS14700	2821967..2822308	-	342	WP_021530985.1	TA system toxin CbtA family protein	Toxin
DV870_RS14705	2822329..2822670	-	342	WP_021530984.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DV870_RS14710	2822681..2823223	-	543	WP_021530983.1	DNA repair protein RadC	-
DV870_RS14715	2823236..2823676	-	441	WP_021530982.1	antirestriction protein	-
DV870_RS14720	2823707..2824528	-	822	Protein_2677	DUF932 domain-containing protein	-
DV870_RS14725	2824649..2825122	-	474	WP_021530979.1	hypothetical protein	-
DV870_RS14730	2825194..2825646	-	453	WP_000734321.1	hypothetical protein	-
DV870_RS14735	2825682..2826398	-	717	WP_001546959.1	WYL domain-containing protein	-
DV870_RS14740	2826642..2827517	-	876	WP_021530978.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	2810066..2842479	32413

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12838.71 Da
• Isoelectric Point: 6.4789

>T108616 WP_021530985.1 NZ_CP031215:c2822308-2821967 [Escherichia coli]
MKTLPATNPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T108616 NZ_CP031215:c2822308-2821967 [Escherichia coli]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCTGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACAGGTTTATTGAAAGCGCG
GCACGGTGAACATTGGGAATAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12712.35 Da
• Isoelectric Point: 6.7345

>AT108616 WP_021530984.1 NZ_CP031215:c2822670-2822329 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT108616 NZ_CP031215:c2822670-2822329 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCGCATTTTATTAGCCAGATGGAATCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACTCCACCCATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0L0H2I3