Detailed information of TA system TA108611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2762556..2763064
• Replicon: chromosome
• Accession: NZ_CP031215
• Organism: Escherichia coli strain Es_ST80_L1_NDM_10_2017

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: DV870_RS14395
• Protein ID: WP_001534843.1
• Coordinates: 2762855..2763064 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: DV870_RS14390
• Protein ID: WP_000063161.1
• Coordinates: 2762556..2762858 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV870_RS14375	2759084..2761234	+	2151	WP_021531004.1	pyruvate/proton symporter BtsT	-
DV870_RS14380	2761352..2761555	+	204	WP_000467859.1	YbdD/YjiX family protein	-
DV870_RS14385	2761566..2762522	+	957	WP_001314406.1	GTPase	-
DV870_RS14390	2762556..2762858	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
DV870_RS14395	2762855..2763064	-	210	WP_001534843.1	BrnT family toxin	Toxin
DV870_RS14400	2763160..2764326	+	1167	WP_000800830.1	restriction endonuclease	-
DV870_RS14405	2764371..2764544	+	174	Protein_2619	SAM-dependent DNA methyltransferase	-
DV870_RS14410	2764538..2765206	+	669	Protein_2620	DUF3387 domain-containing protein	-
-	2765350..2765426	-	77	NuclAT_5	-	-
-	2765350..2765426	-	77	NuclAT_5	-	-
-	2765350..2765426	-	77	NuclAT_5	-	-
-	2765350..2765426	-	77	NuclAT_5	-	-
-	2765350..2765426	-	77	NuclAT_6	-	-
-	2765350..2765426	-	77	NuclAT_6	-	-
-	2765350..2765426	-	77	NuclAT_6	-	-
-	2765350..2765426	-	77	NuclAT_6	-	-
DV870_RS14415	2765421..2765759	+	339	WP_001357525.1	endoribonuclease SymE	-
DV870_RS14420	2765801..2765965	-	165	WP_021531002.1	DUF1127 domain-containing protein	-
DV870_RS14425	2766143..2766466	+	324	Protein_2623	winged helix-turn-helix domain-containing protein	-
DV870_RS14430	2766449..2767369	-	921	WP_021531000.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T108611 WP_001534843.1 NZ_CP031215:c2763064-2762855 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T108611 NZ_CP031215:c2763064-2762855 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT108611 WP_000063161.1 NZ_CP031215:c2762858-2762556 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT108611 NZ_CP031215:c2762858-2762556 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3