Detailed information of TA system TA108587

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3564468..3565147
• Replicon: chromosome
• Accession: NZ_CP031214
• Organism: Escherichia coli strain C600

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: DK885_RS18505
• Protein ID: WP_000854672.1
• Coordinates: 3564468..3564809 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: DK885_RS18510
• Protein ID: WP_000070395.1
• Coordinates: 3564830..3565147 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DK885_RS24045	3560054..3560146	+	93	Protein_3381	sigma factor-binding protein Crl	-
DK885_RS18485	3560185..3561240	-	1056	WP_000749863.1	phosphoporin PhoE	-
DK885_RS18490	3561528..3562631	+	1104	WP_001285288.1	glutamate 5-kinase	-
DK885_RS18495	3562643..3563896	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
DK885_RS18505	3564468..3564809	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
DK885_RS18510	3564830..3565147	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
DK885_RS18515	3565166..3565387	-	222	WP_000691994.1	DUF987 domain-containing protein	-
DK885_RS18520	3565396..3565872	-	477	WP_000811693.1	RadC family protein	-
DK885_RS18525	3565888..3566346	-	459	WP_000211838.1	antirestriction protein	-
DK885_RS18530	3566444..3566683	-	240	WP_000194654.1	DUF905 domain-containing protein	-
DK885_RS18535	3566760..3567227	-	468	WP_001547765.1	hypothetical protein	-
DK885_RS18540	3567250..3567693	-	444	WP_000824223.1	hypothetical protein	-
DK885_RS18545	3567693..3567920	-	228	WP_001548158.1	hypothetical protein	-
DK885_RS18550	3567916..3568107	-	192	Protein_3394	DeoR family transcriptional regulator	-
DK885_RS18555	3568324..3569145	-	822	WP_000197389.1	DUF945 domain-containing protein	-
DK885_RS18560	3569237..3570100	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T108587 WP_000854672.1 NZ_CP031214:c3564809-3564468 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T108587 NZ_CP031214:c3564809-3564468 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT108587 WP_000070395.1 NZ_CP031214:c3565147-3564830 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT108587 NZ_CP031214:c3565147-3564830 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684