Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1845350..1846181 Replicon chromosome
Accession NZ_CP031214
Organism Escherichia coli strain C600

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag DK885_RS09345 Protein ID WP_000854814.1
Coordinates 1845350..1845724 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag DK885_RS09350 Protein ID WP_001285584.1
Coordinates 1845813..1846181 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK885_RS09305 1840746..1841912 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
DK885_RS09310 1842031..1842504 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
DK885_RS09315 1842702..1843760 + 1059 WP_001200891.1 FUSC family protein -
DK885_RS09320 1843932..1844261 + 330 WP_000450409.1 DUF496 family protein -
DK885_RS09325 1844362..1844544 - 183 WP_001016348.1 hypothetical protein -
DK885_RS09330 1844616..1844744 + 129 Protein_1712 transposase domain-containing protein -
DK885_RS09335 1845033..1845146 - 114 WP_001161660.1 DUF957 domain-containing protein -
DK885_RS09340 1845159..1845353 - 195 WP_000988600.1 hypothetical protein -
DK885_RS09345 1845350..1845724 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
DK885_RS09350 1845813..1846181 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
DK885_RS09355 1846255..1846476 - 222 WP_000692323.1 DUF987 domain-containing protein -
DK885_RS09360 1846539..1846985 - 447 WP_000187523.1 RadC family protein -
DK885_RS09365 1846982..1848514 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T108574 WP_000854814.1 NZ_CP031214:c1845724-1845350 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T108574 NZ_CP031214:c1845724-1845350 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT108574 WP_001285584.1 NZ_CP031214:c1846181-1845813 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT108574 NZ_CP031214:c1846181-1845813 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References