Detailed information of TA system TA108560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 2499634..2500199
• Replicon: chromosome
• Accession: NZ_CP031194
• Organism: Streptomyces paludis strain GSSD-12

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DVK44_RS10755
• Protein ID: WP_114659471.1
• Coordinates: 2499634..2500002 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: DVK44_RS10760
• Protein ID: WP_114659472.1
• Coordinates: 2500002..2500199 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVK44_RS10740	2495476..2496270	-	795	WP_114659468.1	hypothetical protein	-
DVK44_RS10745	2496450..2497091	-	642	WP_114659469.1	helix-turn-helix domain-containing protein	-
DVK44_RS10750	2497678..2499471	-	1794	WP_114659470.1	DEAD/DEAH box helicase	-
DVK44_RS10755	2499634..2500002	-	369	WP_114659471.1	Fic family protein	Toxin
DVK44_RS10760	2500002..2500199	-	198	WP_114659472.1	Arc family DNA-binding protein	Antitoxin
DVK44_RS10765	2500274..2501782	-	1509	WP_114659473.1	MFS transporter	-
DVK44_RS10775	2502266..2504569	-	2304	WP_114659474.1	molybdopterin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (12-119)

Antitoxin

• Arc-like DNA binding domain (5-39)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13758.71 Da
• Isoelectric Point: 4.8727

>T108560 WP_114659471.1 NZ_CP031194:c2500002-2499634 [Streptomyces paludis]
MYYLTLPESLGLAKNLGTDEVRDYGLLDSALSRPQASVFGQDAYPDVWQKAAALMESLARNHALVDGNKRIAWYATWVLL
HLNGHPLRPDFDVDEAERFVLSVCQGELEMPKIAELLPRFAR

Nucleotide

• Length: 369 bp

>T108560 NZ_CP031194:c2500002-2499634 [Streptomyces paludis]
ATGTACTACTTGACGCTGCCGGAGAGTCTCGGCCTCGCCAAGAATCTCGGCACCGACGAGGTGCGTGATTACGGGCTGCT
CGACTCGGCCCTCTCGCGCCCGCAGGCGAGCGTGTTCGGCCAGGATGCCTATCCGGACGTATGGCAGAAGGCCGCTGCCC
TGATGGAATCGCTGGCACGCAATCACGCGCTCGTGGACGGCAACAAGCGCATCGCCTGGTATGCAACCTGGGTCCTTCTG
CACCTGAACGGGCATCCGCTGCGACCCGACTTCGACGTCGACGAAGCGGAACGGTTCGTGCTGAGCGTCTGCCAGGGCGA
GCTGGAGATGCCGAAGATCGCTGAGCTGCTCCCGCGCTTCGCGCGCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7315.16 Da
• Isoelectric Point: 5.0637

>AT108560 WP_114659472.1 NZ_CP031194:c2500199-2500002 [Streptomyces paludis]
MAGLNLRFTEEELDALRDRADAEGRSMQSFAHDAVMTAVNERARLFNEAAAHVLRASEELNRRLA

Nucleotide

• Length: 198 bp

>AT108560 NZ_CP031194:c2500199-2500002 [Streptomyces paludis]
ATGGCTGGTCTGAATCTGAGGTTCACCGAAGAAGAGCTGGACGCCCTGCGCGATCGTGCTGATGCTGAGGGGCGCAGTAT
GCAGTCCTTCGCGCACGACGCGGTCATGACCGCTGTGAACGAGCGCGCCCGCCTCTTCAACGAGGCGGCGGCCCACGTGC
TGAGAGCGAGTGAGGAGCTGAACCGGAGGCTCGCCTGA