108559

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 5782..6383 Replicon plasmid pUCCLBBS124_B
Accession NZ_CP031171
Organism Levilactobacillus brevis strain UCCLBBS124

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag UCCLBBS124_RS13130 Protein ID WP_001748110.1
Coordinates 5782..6126 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag UCCLBBS124_RS13135 Protein ID WP_003643337.1
Coordinates 6120..6383 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
UCCLBBS124_RS13100 827..1429 + 603 WP_085763811.1 DUF536 domain-containing protein -
UCCLBBS124_RS13105 1452..2093 + 642 WP_081478640.1 DNA methyltransferase -
UCCLBBS124_RS13115 2208..3911 + 1704 WP_085769742.1 glycosyltransferase family 2 protein -
UCCLBBS124_RS13120 4373..5389 - 1017 WP_014386890.1 IS30 family transposase -
UCCLBBS124_RS13125 5475..5663 + 189 WP_081478641.1 DUF3883 domain-containing protein -
UCCLBBS124_RS13130 5782..6126 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
UCCLBBS124_RS13135 6120..6383 - 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
UCCLBBS124_RS13140 6469..7056 + 588 WP_139566239.1 site-specific integrase -
UCCLBBS124_RS13145 7765..8451 - 687 WP_085769652.1 IS6 family transposase -
UCCLBBS124_RS13150 8569..9024 - 456 WP_086989740.1 hypothetical protein -
UCCLBBS124_RS13155 9035..9331 - 297 WP_086989738.1 hypothetical protein -
UCCLBBS124_RS13160 9399..10424 - 1026 WP_060463087.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..23078 23078


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T108559 WP_001748110.1 NZ_CP031171:c6126-5782 [Levilactobacillus brevis]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T108559 NZ_CP031171:c6126-5782 [Levilactobacillus brevis]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT108559 WP_003643337.1 NZ_CP031171:c6383-6120 [Levilactobacillus brevis]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT108559 NZ_CP031171:c6383-6120 [Levilactobacillus brevis]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGCAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References