Detailed information of TA system TA108557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 253908..254556
• Replicon: chromosome
• Accession: NZ_CP031167
• Organism: Stenotrophomonas sp. ASS1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: MG068_RS01150
• Protein ID: WP_132808853.1
• Coordinates: 253908..254195 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A0L8AF77
• Locus tag: MG068_RS01155
• Protein ID: WP_029380112.1
• Coordinates: 254254..254556 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MG068_RS01120	249170..250138	+	969	WP_132808845.1	bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA	-
MG068_RS01125	250135..250866	+	732	WP_005407698.1	type III pantothenate kinase	-
MG068_RS01130	250876..251724	+	849	WP_132808847.1	SPOR domain-containing protein	-
MG068_RS01140	251925..253049	-	1125	WP_132808849.1	hypothetical protein	-
MG068_RS01145	253077..253676	-	600	WP_132808851.1	hypothetical protein	-
MG068_RS01150	253908..254195	+	288	WP_132808853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MG068_RS01155	254254..254556	+	303	WP_029380112.1	putative addiction module antidote protein	Antitoxin
MG068_RS01160	254732..255133	-	402	WP_132808855.1	hypothetical protein	-
MG068_RS01165	255730..256500	+	771	WP_132808857.1	DUF3011 domain-containing protein	-
MG068_RS01170	256559..257503	+	945	WP_132808859.1	ankyrin repeat domain-containing protein	-
MG068_RS01175	257521..257868	-	348	WP_132808861.1	hypothetical protein	-
MG068_RS01180	257999..258919	+	921	WP_094001609.1	arginase	-
MG068_RS01185	259082..259219	+	138	WP_008266021.1	entericidin A/B family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	237557..255133	17576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-95)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10716.33 Da
• Isoelectric Point: 10.7813

>T108557 WP_132808853.1 NZ_CP031167:253908-254195 [Stenotrophomonas sp. ASS1]
MKIQRTRQFATWIDALKGVTARARILARIGRLAEGHPDDHRYLADGVSELRIDAGPGYRVYYTQRGRQLVILLVGGDKGS
QQRDIEKAKEIARAL

Nucleotide

• Length: 288 bp

>T108557 NZ_CP031167:253908-254195 [Stenotrophomonas sp. ASS1]
ATGAAGATCCAGCGTACCCGTCAGTTCGCGACATGGATTGACGCTCTCAAGGGCGTAACCGCACGCGCTCGCATCCTGGC
CCGTATCGGTCGGCTCGCTGAAGGCCATCCCGATGATCACCGCTATCTGGCGGATGGCGTTTCTGAATTGCGTATCGATG
CAGGGCCGGGTTACCGCGTTTACTACACCCAGCGCGGCAGGCAGTTGGTGATTCTCCTGGTTGGAGGCGACAAGGGTTCG
CAGCAGCGCGATATCGAGAAGGCCAAGGAGATTGCGCGTGCCCTGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10750.33 Da
• Isoelectric Point: 8.0359

>AT108557 WP_029380112.1 NZ_CP031167:254254-254556 [Stenotrophomonas sp. ASS1]
MAIKRKVELKSFDVAEHLRTPEEMAAYLDACIEESDGDSAFIAKALGDIARAQGMSKVARAAGLSRESLYRALSGERSPD
FATILKVTRALGVRLHASAA

Nucleotide

• Length: 303 bp

>AT108557 NZ_CP031167:254254-254556 [Stenotrophomonas sp. ASS1]
ATGGCCATCAAGAGAAAAGTCGAACTCAAATCATTTGATGTGGCCGAGCACCTTCGGACACCGGAGGAGATGGCGGCCTA
TCTCGATGCCTGCATCGAGGAAAGCGATGGCGATTCCGCGTTCATTGCCAAGGCGCTCGGCGACATCGCGCGCGCTCAGG
GCATGAGCAAGGTTGCGCGTGCGGCGGGCTTGTCGCGCGAGAGTCTGTATCGCGCACTGTCGGGCGAGCGCAGCCCGGAT
TTCGCGACCATCCTGAAGGTGACCCGGGCCCTGGGTGTGCGATTGCATGCCAGCGCAGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0L8AF77