Detailed information of TA system TA108553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 621900..622489
• Replicon: plasmid pDrdI
• Accession: NZ_CP031163
• Organism: Deinococcus wulumuqiensis strain NEB 479

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DVJ83_RS18630
• Protein ID: WP_114673729.1
• Coordinates: 621900..622232 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: DVJ83_RS18635
• Protein ID: WP_114673730.1
• Coordinates: 622232..622489 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVJ83_RS18615	619077..619787	-	711	WP_114673726.1	IS6 family transposase	-
DVJ83_RS18620	619808..620467	-	660	WP_162865882.1	metallophosphoesterase	-
DVJ83_RS18625	620489..621613	-	1125	WP_114673728.1	transposase	-
DVJ83_RS18630	621900..622232	-	333	WP_114673729.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DVJ83_RS18635	622232..622489	-	258	WP_114673730.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DVJ83_RS18640	622627..623097	-	471	WP_114673731.1	DUF4326 domain-containing protein	-
DVJ83_RS18645	623277..623711	-	435	WP_205413570.1	DUF1768 domain-containing protein	-
DVJ83_RS18650	623811..624131	-	321	WP_114673733.1	hypothetical protein	-
DVJ83_RS18655	624230..624658	-	429	WP_114673734.1	hypothetical protein	-
DVJ83_RS18660	624734..625297	-	564	WP_114673735.1	Fic family protein	-
DVJ83_RS18665	625278..625469	-	192	WP_114673736.1	hypothetical protein	-
DVJ83_RS18670	625594..626304	-	711	WP_114673737.1	IS6 family transposase	-
DVJ83_RS18675	626445..626705	+	261	WP_114673738.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
DVJ83_RS18680	626706..627098	+	393	WP_114673739.1	PIN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..668667	668667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11727.45 Da
• Isoelectric Point: 9.2719

>T108553 WP_114673729.1 NZ_CP031163:c622232-621900 [Deinococcus wulumuqiensis]
MERGDIYFVSLDPTAGHEQRGSRPVLVVSPGAFNALTGTPIVLPITNGGQFARTAGFAVSLEGTGIRTTGVIRCDQPRAV
DLKARQGRHVERLPDTVMAEVLARLSTLLS

Nucleotide

• Length: 333 bp

>T108553 NZ_CP031163:c622232-621900 [Deinococcus wulumuqiensis]
ATGGAGCGCGGCGACATCTATTTCGTCAGCCTGGACCCCACCGCCGGGCACGAGCAACGCGGCAGCCGCCCGGTCCTGGT
CGTGTCACCGGGAGCGTTCAACGCCCTGACGGGGACGCCGATTGTCCTGCCCATCACGAATGGCGGCCAGTTCGCGCGCA
CGGCCGGATTTGCGGTCTCGCTGGAAGGGACAGGTATCCGGACAACCGGCGTAATCCGCTGTGACCAGCCGCGCGCCGTG
GACCTGAAGGCCCGCCAGGGTCGCCACGTGGAACGCCTTCCCGACACCGTGATGGCGGAGGTGCTGGCCCGACTCTCGAC
CCTCCTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9162.64 Da
• Isoelectric Point: 4.2965

>AT108553 WP_114673730.1 NZ_CP031163:c622489-622232 [Deinococcus wulumuqiensis]
MHTTQLRKVGGSIMLRIPPALLDVLHLNAGATVDLTLEDGRLIVAPVPVPRYTLADLLAECDPALPLTDEDRQWLDAPAA
GNELL

Nucleotide

• Length: 258 bp

>AT108553 NZ_CP031163:c622489-622232 [Deinococcus wulumuqiensis]
ATGCACACCACCCAACTGCGTAAAGTCGGCGGTTCCATCATGCTCCGCATCCCCCCGGCTCTGCTCGACGTCCTGCACCT
GAACGCAGGCGCGACCGTGGATCTGACCCTGGAGGACGGCCGCCTCATCGTCGCGCCTGTCCCCGTGCCCCGGTACACCC
TGGCGGACCTGCTTGCCGAGTGTGATCCTGCGTTGCCCCTGACCGACGAGGACCGCCAGTGGCTGGACGCCCCTGCCGCT
GGAAATGAACTCCTGTAA