Detailed information of TA system TA108550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-AbrB
• Location: 2162720..2163366
• Replicon: chromosome
• Accession: NZ_CP031156
• Organism: Metallosphaera prunae strain Ron 12/II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A088E7Y6
• Locus tag: DFR88_RS11810
• Protein ID: WP_012021252.1
• Coordinates: 2162977..2163366 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DFR88_RS11805
• Protein ID: WP_012021253.1
• Coordinates: 2162720..2162980 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DFR88_RS11775 (DFR88_11750)	2157829..2158281	-	453	WP_012021259.1	peroxiredoxin	-
DFR88_RS11780 (DFR88_11755)	2158513..2159085	+	573	WP_012021258.1	hypothetical protein	-
DFR88_RS11785 (DFR88_11760)	2159100..2160452	-	1353	WP_193453306.1	APC family permease	-
DFR88_RS11790 (DFR88_11765)	2160638..2161147	-	510	WP_012021256.1	OsmC family protein	-
DFR88_RS11795 (DFR88_11770)	2161149..2161799	-	651	WP_012021255.1	cyclase family protein	-
DFR88_RS11800 (DFR88_11775)	2162028..2162654	+	627	WP_048060318.1	type 1 glutamine amidotransferase	-
DFR88_RS11805 (DFR88_11780)	2162720..2162980	+	261	WP_012021253.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DFR88_RS11810 (DFR88_11785)	2162977..2163366	+	390	WP_012021252.1	type II toxin-antitoxin system VapC family toxin	Toxin
DFR88_RS11815 (DFR88_11790)	2163423..2164586	+	1164	WP_012021251.1	glycosyltransferase	-
DFR88_RS11820 (DFR88_11795)	2164679..2165722	+	1044	WP_012021250.1	HoxN/HupN/NixA family nickel/cobalt transporter	-
DFR88_RS11825 (DFR88_11800)	2165757..2166380	+	624	WP_012021249.1	PIG-L family deacetylase	-
DFR88_RS11830 (DFR88_11805)	2166449..2167630	-	1182	WP_193453307.1	mandelate racemase/muconate lactonizing enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 15075.63 Da
• Isoelectric Point: 4.9164

>T108550 WP_012021252.1 NZ_CP031156:2162977-2163366 [Metallosphaera prunae]
MIFLDANFLVYLNLGVDPVKSFYLRILSNESMGVDPLVLDEVIYVSRKKYGVNFGDTLRFLDELVLPYVVVFPISLREYQ
VSKELITRYSLYPSDAFHVAVMLNNSIKKILTEDSDFEKIKEIERIWIK

Nucleotide

• Length: 390 bp

>T108550 NZ_CP031156:2162977-2163366 [Metallosphaera prunae]
TTGATATTCTTGGATGCAAACTTCTTGGTGTACCTAAACCTGGGAGTTGACCCGGTCAAGAGCTTCTACCTCAGGATACT
TTCCAATGAGAGTATGGGCGTTGACCCCCTGGTCCTAGACGAGGTGATCTACGTCTCGAGGAAGAAGTATGGCGTGAATT
TCGGCGACACATTGCGATTTCTGGACGAGCTAGTCTTACCTTACGTAGTGGTATTTCCGATCTCCCTGAGGGAGTACCAG
GTTAGCAAGGAGCTCATCACTAGGTATTCCCTCTATCCCTCAGACGCGTTTCACGTGGCAGTAATGCTCAACAACTCCAT
AAAGAAGATTCTCACCGAGGACTCCGACTTCGAGAAGATCAAGGAAATAGAGAGGATCTGGATAAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10073.56 Da
• Isoelectric Point: 4.8329

>AT108550 WP_012021253.1 NZ_CP031156:2162720-2162980 [Metallosphaera prunae]
MTLRVEVGKKGYMIIPKNVRDLLGINEGDFLELRVENGKIVLEKERKVDLEEVERKFEEHERRIAYARRASLGDLKGVVL
EEEFDD

Nucleotide

• Length: 261 bp

>AT108550 NZ_CP031156:2162720-2162980 [Metallosphaera prunae]
TTGACTCTTAGGGTAGAGGTAGGAAAGAAGGGTTACATGATAATCCCCAAGAACGTCAGAGACCTGCTCGGCATCAATGA
GGGCGACTTCCTGGAACTCAGGGTAGAGAATGGCAAGATAGTCCTGGAAAAGGAAAGGAAAGTTGATCTGGAGGAGGTGG
AGAGGAAGTTTGAGGAACACGAGAGAAGGATCGCGTATGCAAGAAGGGCCAGCCTTGGAGATTTAAAGGGCGTCGTGTTG
GAGGAGGAGTTTGACGATTGA

Structures

Toxin

• AlphaFold DB: A0A088E7Y6