Detailed information of TA system TA108548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 4421338..4421927
• Replicon: chromosome
• Accession: NZ_CP031146
• Organism: Pseudomonas plecoglossicida strain XSDHY-P

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DVB73_RS20360
• Protein ID: WP_028625322.1
• Coordinates: 4421338..4421634 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: S2KHC3
• Locus tag: DVB73_RS20365
• Protein ID: WP_016393973.1
• Coordinates: 4421631..4421927 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVB73_RS20335	4416960..4417814	+	855	WP_016393967.1	putative selenate ABC transporter substrate-binding protein	-
DVB73_RS20340	4417811..4418605	+	795	WP_016393968.1	phosphonate ABC transporter ATP-binding protein	-
DVB73_RS20345	4418599..4419426	+	828	WP_016393969.1	ABC transporter permease	-
DVB73_RS20350	4419423..4420190	+	768	WP_016393970.1	phosphonate ABC transporter, permease protein PhnE	-
DVB73_RS20355	4420225..4421115	-	891	WP_016393971.1	hypothetical protein	-
DVB73_RS20360	4421338..4421634	+	297	WP_028625322.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DVB73_RS20365	4421631..4421927	+	297	WP_016393973.1	putative addiction module antidote protein	Antitoxin
DVB73_RS20370	4421933..4422418	-	486	WP_016393974.1	GNAT family N-acetyltransferase	-
DVB73_RS20375	4422502..4423149	-	648	WP_028625324.1	hypothetical protein	-
DVB73_RS20385	4423614..4424663	+	1050	WP_016393976.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
DVB73_RS20390	4424678..4425793	+	1116	WP_016393977.1	tRNA guanosine(34) transglycosylase Tgt	-
DVB73_RS20395	4425837..4426172	+	336	WP_008092587.1	preprotein translocase subunit YajC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (41-87)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10910.84 Da
• Isoelectric Point: 10.9027

>T108548 WP_028625322.1 NZ_CP031146:4421338-4421634 [Pseudomonas plecoglossicida]
MICVVEQTEHFARWLAGLKDLRAKLAVGRRIERAAMGNLGDFKSVGGSISELRVDVAGGYRIYFTRKQHRLIILLVGGDK
SSQATDILKARKLAKELK

Nucleotide

• Length: 297 bp

>T108548 NZ_CP031146:4421338-4421634 [Pseudomonas plecoglossicida]
ATGATCTGCGTTGTTGAGCAAACCGAGCACTTCGCTCGCTGGTTGGCGGGCTTGAAAGACCTCAGGGCCAAACTGGCTGT
CGGTCGGAGGATCGAACGAGCGGCGATGGGTAATCTGGGCGACTTCAAGTCAGTTGGAGGAAGCATCAGTGAACTTCGCG
TCGACGTCGCTGGGGGGTACCGGATCTACTTCACGCGAAAGCAACACCGGTTGATCATTTTGCTTGTTGGAGGGGACAAA
TCCTCTCAGGCCACCGATATCCTGAAGGCCAGAAAGCTGGCAAAGGAGCTGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10536.12 Da
• Isoelectric Point: 5.6706

>AT108548 WP_016393973.1 NZ_CP031146:4421631-4421927 [Pseudomonas plecoglossicida]
MNEKLIPFDMVTLLDSDEAISEYLSQVLADGDTDEIIRALGHIARARGMSQIAAGSGLGRESLYKALTPGAKPRFDTVLK
VIRALGIDLSARPHLVSS

Nucleotide

• Length: 297 bp

>AT108548 NZ_CP031146:4421631-4421927 [Pseudomonas plecoglossicida]
ATGAACGAGAAGCTCATTCCTTTTGACATGGTCACACTGCTGGACAGCGACGAAGCTATCAGCGAGTACCTGAGTCAGGT
CCTGGCCGATGGCGACACGGATGAAATCATTCGTGCGCTCGGCCACATTGCAAGGGCGCGTGGAATGTCTCAGATTGCAG
CCGGTAGTGGTTTGGGAAGAGAGAGCCTTTACAAGGCCCTCACACCTGGGGCAAAGCCCCGCTTTGATACAGTACTGAAG
GTCATTCGGGCGCTTGGCATCGATCTTTCTGCGCGGCCTCACCTGGTTTCGAGCTGA

Structures

Antitoxin

• AlphaFold DB: S2KHC3