Detailed information of TA system TA108547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3355936..3357665
• Replicon: chromosome
• Accession: NZ_CP031146
• Organism: Pseudomonas plecoglossicida strain XSDHY-P

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: DVB73_RS15390
• Protein ID: WP_028625067.1
• Coordinates: 3357024..3357665 (-)
• Length: 214 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: S2K0E1
• Locus tag: DVB73_RS15385
• Protein ID: WP_016393333.1
• Coordinates: 3355936..3357024 (-)
• Length: 363 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVB73_RS15370	3351338..3352393	-	1056	WP_016393336.1	magnesium transporter	-
DVB73_RS15375	3352639..3353715	-	1077	WP_043172821.1	phosphatidylinositol-specific phospholipase C	-
DVB73_RS15380	3354036..3355877	+	1842	WP_016393334.1	dihydroxy-acid dehydratase	-
DVB73_RS15385	3355936..3357024	-	1089	WP_016393333.1	macro domain-containing protein	Antitoxin
DVB73_RS15390	3357024..3357665	-	642	WP_028625067.1	DUF4433 domain-containing protein	Toxin
DVB73_RS15395	3357662..3358240	-	579	WP_016393331.1	DUF4433 domain-containing protein	-
DVB73_RS15400	3358329..3359384	-	1056	WP_016393330.1	haloacid dehalogenase-like hydrolase	-
DVB73_RS15405	3359626..3361017	+	1392	WP_016393329.1	L-cystine transporter	-
DVB73_RS15410	3361054..3361569	-	516	WP_016393328.1	dihydrofolate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 214 a.a.
• Molecular weight: 24628.20 Da
• Isoelectric Point: 9.1292

>T108547 WP_028625067.1 NZ_CP031146:c3357665-3357024 [Pseudomonas plecoglossicida]
MIYSSLNPSKALIWRIVHRDNLPWLLDHGLHAVSSGRMSSTYVGIGNPELISKRAQRDVPIEPNGTLADYVPFYFTPFSV
MMKNIHSGWSVQQRRNEEIVILVSSLYHAQSLGLRFVFTDAHAYPHWTGYYSDLAQLDKIDWEILQRRDFKRDPDDPRKM
ERYQAEALIHGHVPVAGLLGIVCYNEALKSRVVKEVQQRQLSLPVHARPGWYF

Nucleotide

• Length: 642 bp

>T108547 NZ_CP031146:c3357665-3357024 [Pseudomonas plecoglossicida]
ATGATCTACTCCAGTCTCAACCCATCCAAGGCGCTCATCTGGCGAATTGTGCACCGCGATAATCTGCCGTGGCTGCTGGA
TCATGGGCTGCACGCGGTCTCTTCAGGAAGGATGTCCAGTACCTACGTCGGGATCGGCAATCCAGAGCTGATCAGCAAGC
GAGCTCAACGCGATGTGCCTATAGAGCCCAACGGAACCCTTGCAGATTATGTGCCTTTTTACTTCACTCCGTTCTCAGTG
ATGATGAAGAACATTCATTCGGGGTGGAGTGTGCAGCAGCGGCGGAACGAAGAGATCGTGATTCTTGTCTCCAGCCTGTA
CCATGCGCAGTCGCTAGGTCTGCGTTTTGTCTTCACGGATGCCCATGCCTACCCGCATTGGACCGGCTACTACAGCGATC
TCGCGCAGTTGGACAAAATCGATTGGGAGATTTTGCAGCGCAGGGACTTCAAACGTGATCCTGATGATCCTCGAAAAATG
GAACGTTATCAGGCTGAAGCCCTTATCCATGGACATGTCCCGGTCGCTGGTCTTCTGGGAATTGTCTGCTACAACGAAGC
TTTGAAGAGTCGTGTCGTCAAGGAGGTTCAGCAGCGTCAACTGAGCCTCCCCGTGCATGCTCGCCCTGGGTGGTACTTCT
GA

Antitoxin

Protein

• Length: 363 a.a.
• Molecular weight: 40514.55 Da
• Isoelectric Point: 5.8940

>AT108547 WP_016393333.1 NZ_CP031146:c3357024-3355936 [Pseudomonas plecoglossicida]
MIRFMQGNLLEAKADALVNTVNTVGVMGKGIALMFKERFTENYRQYAAACKAKEVVTGKMFVTEVRELDGPRWIVNFPTK
RHWRSPSQIGWIVDGLHDLRSFIIENQVVSIAIPPLGAGNGGLPWTAVREQIESILKDLDADILVYEPTANYQNVSKRSG
VENLTPARALIAELIRRYWVLGMECSLLEVQKLAWFLERSIERFTPGVNPLDLKFVAYKYGPYANRLDHLLDNLDGSYLH
CGKRISDAGPLDVIWFDEGRKAFVQAYLNSEAKVHLPALEFTADLIDGFESPFGMELLATVDWLLHEDGVEPTVPAVREH
LKNWPGGAGAAARKSKLFDDDSLGIALEKLSMFRSDPAASLH

Nucleotide

• Length: 1089 bp

>AT108547 NZ_CP031146:c3357024-3355936 [Pseudomonas plecoglossicida]
ATGATTCGCTTCATGCAAGGTAACCTGCTGGAGGCCAAGGCCGACGCGCTGGTTAATACAGTCAACACCGTAGGGGTGAT
GGGGAAAGGCATTGCGCTGATGTTCAAGGAGCGCTTTACCGAGAACTATCGCCAGTACGCTGCTGCCTGCAAAGCGAAAG
AAGTAGTTACTGGGAAGATGTTTGTTACTGAAGTGCGTGAGCTGGATGGGCCGCGCTGGATTGTGAATTTTCCAACCAAG
CGGCATTGGCGCTCACCTTCACAAATTGGCTGGATCGTCGATGGGCTCCATGATCTGCGCAGCTTTATTATTGAGAATCA
AGTAGTTTCGATTGCTATCCCTCCACTTGGTGCTGGTAATGGAGGGTTGCCTTGGACTGCTGTACGAGAGCAGATTGAAA
GCATTCTGAAAGATTTGGATGCGGATATTCTCGTTTATGAGCCTACTGCAAATTATCAAAACGTTTCCAAGCGCAGCGGT
GTTGAGAACCTGACGCCTGCTCGTGCGCTGATTGCAGAGCTGATACGCCGCTATTGGGTGTTGGGGATGGAGTGCAGCTT
GCTCGAAGTTCAAAAGCTCGCCTGGTTTTTGGAGCGATCAATCGAGAGGTTCACGCCGGGCGTCAACCCATTGGACTTGA
AGTTTGTCGCTTACAAATATGGCCCTTATGCCAACCGGTTGGACCACCTTCTAGACAATCTTGATGGCAGTTATTTGCAC
TGTGGGAAACGGATCAGCGATGCAGGCCCTCTGGACGTAATCTGGTTCGATGAGGGGCGCAAGGCATTTGTACAGGCATA
TTTGAACAGTGAGGCCAAGGTTCATTTGCCTGCATTGGAGTTCACCGCTGATTTGATCGATGGCTTTGAATCTCCCTTTG
GGATGGAGCTGCTTGCCACTGTTGATTGGCTATTGCATGAAGATGGTGTTGAGCCGACAGTCCCTGCTGTTCGAGAGCAT
TTGAAGAATTGGCCTGGAGGTGCGGGGGCCGCAGCCCGGAAAAGCAAATTGTTCGATGATGATTCTCTTGGGATCGCTCT
TGAGAAGTTATCAATGTTTCGTTCGGATCCTGCCGCTTCACTTCATTGA

Structures

Antitoxin

• AlphaFold DB: S2K0E1