Detailed information of TA system TA108544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3603952..3604493
• Replicon: chromosome
• Accession: NZ_CP031145
• Organism: Intrasporangium calvum strain C5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DN585_RS16580
• Protein ID: WP_013494289.1
• Coordinates: 3603952..3604230 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6SG24
• Locus tag: DN585_RS16585
• Protein ID: WP_013494290.1
• Coordinates: 3604227..3604493 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN585_RS16550	3599036..3600178	+	1143	WP_013494283.1	zinc-binding dehydrogenase	-
DN585_RS16555	3600455..3600793	-	339	WP_013494284.1	type II toxin-antitoxin system PemK/MazF family toxin	-
DN585_RS16560	3600777..3600974	-	198	WP_013494285.1	hypothetical protein	-
DN585_RS16565	3601166..3601654	+	489	WP_148236665.1	hypothetical protein	-
DN585_RS16570	3601985..3603280	+	1296	WP_013494287.1	IS110 family transposase	-
DN585_RS16575	3603350..3603814	-	465	WP_052338053.1	cupin domain-containing protein	-
DN585_RS16580	3603952..3604230	-	279	WP_013494289.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DN585_RS16585	3604227..3604493	-	267	WP_013494290.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DN585_RS16590	3604664..3605578	-	915	WP_114610707.1	tyrosine-type recombinase/integrase	-
DN585_RS16595	3605783..3606187	+	405	WP_013494293.1	NUDIX domain-containing protein	-
DN585_RS16600	3606289..3606999	-	711	WP_148236669.1	DUF429 domain-containing protein	-
DN585_RS16605	3607366..3607590	-	225	WP_013494295.1	hypothetical protein	-
DN585_RS16610	3607766..3608389	+	624	WP_013494296.1	GNAT family N-acetyltransferase	-
DN585_RS19025	3608549..3608722	-	174	WP_169312936.1	hypothetical protein	-
DN585_RS16615	3609012..3609443	+	432	WP_114610708.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3592869..3613837	20968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (31-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.80 Da
• Isoelectric Point: 10.3818

>T108544 WP_013494289.1 NZ_CP031145:c3604230-3603952 [Intrasporangium calvum]
VSEEPYELVLTPPAVRAVRSGLPEAVAAAVIEFLTGALIQNPHRVGKPLRGELAGIHSARRGTYRVLYRINEIQGEVVVL
RIDHRRDAYRRG

Nucleotide

• Length: 279 bp

>T108544 NZ_CP031145:c3604230-3603952 [Intrasporangium calvum]
GTGAGCGAAGAACCGTACGAACTGGTCCTGACCCCTCCCGCCGTCCGAGCCGTGCGCTCAGGACTGCCCGAGGCCGTTGC
GGCGGCGGTCATAGAGTTCCTCACCGGAGCATTGATCCAGAACCCGCACCGCGTCGGCAAGCCATTGCGGGGGGAGCTCG
CGGGTATCCACTCGGCCCGGCGCGGCACCTACCGCGTCCTGTATCGAATCAACGAGATCCAAGGCGAAGTGGTGGTTCTC
CGCATCGACCACCGACGAGACGCTTACCGACGCGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9717.81 Da
• Isoelectric Point: 4.4325

>AT108544 WP_013494290.1 NZ_CP031145:c3604493-3604227 [Intrasporangium calvum]
MTVEPLRDVRNHFSDVIDRVEHEHERVTVTRNGRPVAVILSPDDLAQLEETLSVLSDPEALADIREADAAYATGDVVRGV
EAVRGLRS

Nucleotide

• Length: 267 bp

>AT108544 NZ_CP031145:c3604493-3604227 [Intrasporangium calvum]
ATGACTGTTGAACCGCTTCGCGATGTCCGCAACCATTTCAGCGACGTCATCGACCGCGTCGAGCATGAGCACGAGCGGGT
CACAGTCACGCGCAATGGACGCCCGGTTGCCGTCATCCTCAGCCCGGACGACCTGGCGCAGCTGGAGGAGACCCTCTCGG
TGCTCAGCGACCCCGAAGCCCTCGCCGACATCCGTGAGGCCGATGCGGCATACGCGACCGGTGACGTCGTTCGCGGGGTC
GAGGCCGTGCGCGGCCTGCGCTCGTGA

Structures

Antitoxin

• AlphaFold DB: E6SG24