Detailed information of TA system TA108541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 2063813..2064366
• Replicon: chromosome
• Accession: NZ_CP031145
• Organism: Intrasporangium calvum strain C5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DN585_RS09450
• Protein ID: WP_052338114.1
• Coordinates: 2063813..2064037 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6SCZ8
• Locus tag: DN585_RS09455
• Protein ID: WP_013492901.1
• Coordinates: 2064085..2064366 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN585_RS09420	2059432..2059773	-	342	WP_013492896.1	bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit	-
DN585_RS09425	2059858..2060517	-	660	WP_114609968.1	metalloregulator ArsR/SmtB family transcription factor	-
DN585_RS09430	2060514..2061824	-	1311	WP_114611048.1	MFS transporter	-
DN585_RS09440	2062547..2062867	-	321	WP_199921493.1	transposase	-
DN585_RS09445	2063387..2063698	+	312	WP_041307550.1	hypothetical protein	-
DN585_RS09450	2063813..2064037	-	225	WP_052338114.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DN585_RS09455	2064085..2064366	-	282	WP_013492901.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DN585_RS09460	2064797..2065270	+	474	WP_013492902.1	hypothetical protein	-
DN585_RS09465	2065280..2065633	+	354	WP_114609970.1	hypothetical protein	-
DN585_RS09470	2065888..2066244	-	357	WP_114609971.1	metalloregulator ArsR/SmtB family transcription factor	-
DN585_RS09475	2066414..2067508	+	1095	WP_199921717.1	ACR3 family arsenite efflux transporter	-
DN585_RS09480	2067501..2067947	+	447	WP_114609973.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (20-72)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8371.53 Da
• Isoelectric Point: 8.5956

>T108541 WP_052338114.1 NZ_CP031145:c2064037-2063813 [Intrasporangium calvum]
MTEDLPEAVATAAFEFITGPLLENPHRVGKQLRPPLTDRHSARRGTYRILYRIDEQAQTVTVLAIGHRGDIYRA

Nucleotide

• Length: 225 bp

>T108541 NZ_CP031145:c2064037-2063813 [Intrasporangium calvum]
ATGACTGAAGATCTCCCAGAAGCCGTCGCCACCGCCGCCTTCGAGTTCATCACCGGCCCCTTGCTCGAGAACCCTCACCG
CGTGGGCAAGCAACTGCGCCCACCCCTGACAGACCGACACAGCGCACGACGTGGCACCTATCGCATCCTCTACCGCATCG
ACGAACAAGCCCAGACGGTGACCGTCCTAGCGATCGGCCACCGTGGCGACATCTACCGTGCGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 9902.22 Da
• Isoelectric Point: 5.0600

>AT108541 WP_013492901.1 NZ_CP031145:c2064366-2064085 [Intrasporangium calvum]
MTSIPLADAKAHLSEVISRVSKHHERVTVTVHGQPSAVLLAPDDLAMLEETIAVLGDRDLMTQLATADADLAAGRVETQG
DLEAAMRSRRTSA

Nucleotide

• Length: 282 bp

>AT108541 NZ_CP031145:c2064366-2064085 [Intrasporangium calvum]
ATGACGAGCATTCCACTGGCCGACGCCAAGGCACACCTGTCCGAGGTCATCAGTCGAGTGAGCAAGCACCACGAACGAGT
AACCGTGACCGTGCACGGTCAGCCCTCGGCCGTCCTGCTGGCGCCCGATGACCTCGCGATGCTCGAGGAGACCATCGCCG
TCTTGGGGGACCGCGACCTCATGACGCAGCTTGCCACCGCCGACGCCGACCTGGCTGCCGGCCGTGTCGAGACCCAAGGC
GACCTCGAGGCGGCGATGCGGTCACGCCGGACCTCGGCGTGA

Structures

Antitoxin

• AlphaFold DB: E6SCZ8