Detailed information of TA system TA108540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 756133..756686
• Replicon: chromosome
• Accession: NZ_CP031145
• Organism: Intrasporangium calvum strain C5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DN585_RS03545
• Protein ID: WP_052338114.1
• Coordinates: 756462..756686 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6SCZ8
• Locus tag: DN585_RS03540
• Protein ID: WP_013492901.1
• Coordinates: 756133..756414 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN585_RS03500	751484..751891	+	408	WP_114609445.1	hypothetical protein	-
DN585_RS03505	752012..752257	-	246	WP_013491716.1	hypothetical protein	-
DN585_RS03510	752268..752504	-	237	WP_013491717.1	BrnT family toxin	-
DN585_RS03515	752596..753249	-	654	WP_150132565.1	META domain-containing protein	-
DN585_RS03520	753246..753746	-	501	WP_114610977.1	SigE family RNA polymerase sigma factor	-
DN585_RS03525	753989..754933	+	945	WP_114609447.1	cold shock domain-containing protein	-
DN585_RS03530	755043..755282	-	240	WP_114609448.1	hypothetical protein	-
DN585_RS03535	755461..755964	-	504	WP_150132567.1	hypothetical protein	-
DN585_RS03540	756133..756414	+	282	WP_013492901.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DN585_RS03545	756462..756686	+	225	WP_052338114.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DN585_RS03550	756837..757082	+	246	WP_150132568.1	nucleotidyltransferase family protein	-
DN585_RS03555	757163..758158	+	996	WP_199921638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (20-72)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8371.53 Da
• Isoelectric Point: 8.5956

>T108540 WP_052338114.1 NZ_CP031145:756462-756686 [Intrasporangium calvum]
MTEDLPEAVATAAFEFITGPLLENPHRVGKQLRPPLTDRHSARRGTYRILYRIDEQAQTVTVLAIGHRGDIYRA

Nucleotide

• Length: 225 bp

>T108540 NZ_CP031145:756462-756686 [Intrasporangium calvum]
ATGACTGAAGATCTCCCAGAAGCCGTCGCCACCGCCGCCTTCGAGTTCATCACCGGCCCCTTGCTCGAGAACCCTCACCG
CGTGGGCAAGCAACTGCGCCCACCCCTGACAGACCGACACAGCGCACGACGTGGCACCTATCGCATCCTCTACCGCATCG
ACGAACAAGCCCAGACGGTAACCGTCCTAGCGATCGGCCACCGTGGCGACATCTACCGTGCGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 9902.22 Da
• Isoelectric Point: 5.0600

>AT108540 WP_013492901.1 NZ_CP031145:756133-756414 [Intrasporangium calvum]
MTSIPLADAKAHLSEVISRVSKHHERVTVTVHGQPSAVLLAPDDLAMLEETIAVLGDRDLMTQLATADADLAAGRVETQG
DLEAAMRSRRTSA

Nucleotide

• Length: 282 bp

>AT108540 NZ_CP031145:756133-756414 [Intrasporangium calvum]
ATGACGAGCATTCCACTGGCCGACGCCAAGGCACACCTGTCCGAGGTCATCAGCCGAGTGAGCAAGCACCACGAACGAGT
AACCGTGACCGTGCACGGTCAGCCCTCGGCCGTCCTGCTGGCGCCCGATGACCTCGCGATGCTCGAGGAGACCATTGCCG
TCTTGGGGGACCGCGACCTCATGACGCAGCTTGCCACCGCCGACGCCGACCTGGCTGCCGGCCGTGTCGAGACCCAAGGC
GACCTCGAGGCGGCGATGCGGTCACGCCGGACCTCGGCGTGA

Structures

Antitoxin

• AlphaFold DB: E6SCZ8