Detailed information of TA system TA108536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 5197341..5197905
• Replicon: chromosome
• Accession: NZ_CP031142
• Organism: Saccharopolyspora pogona strain NRRL30141

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DL519_RS23890
• Protein ID: WP_190818084.1
• Coordinates: 5197558..5197905 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DL519_RS23885
• Protein ID: WP_190818082.1
• Coordinates: 5197341..5197571 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL519_RS23865	5193724..5194614	-	891	WP_190818077.1	nucleotide exchange factor GrpE	-
DL519_RS23870	5194895..5195470	-	576	WP_190818079.1	snapalysin family zinc-dependent metalloprotease	-
DL519_RS23875	5195863..5196597	+	735	WP_190818080.1	PIG-L family deacetylase	-
DL519_RS23880	5196594..5197217	+	624	WP_190818081.1	maleylpyruvate isomerase N-terminal domain-containing protein	-
DL519_RS23885	5197341..5197571	+	231	WP_190818082.1	ribbon-helix-helix domain-containing protein	Antitoxin
DL519_RS23890	5197558..5197905	+	348	WP_190818084.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DL519_RS23895	5198076..5199011	-	936	WP_168586883.1	heme o synthase	-
DL519_RS23900	5199008..5199406	-	399	WP_190818086.1	DUF3817 domain-containing protein	-
DL519_RS23905	5199721..5200815	-	1095	WP_190818088.1	LLM class flavin-dependent oxidoreductase	-
DL519_RS23910	5201126..5201293	-	168	Protein_4763	SidA/IucD/PvdA family monooxygenase	-
DL519_RS23915	5201576..5201746	-	171	WP_190824175.1	hypothetical protein	-
DL519_RS23920	5201792..5202145	-	354	WP_190818090.1	MFS transporter	-
DL519_RS23925	5202145..5202333	-	189	WP_190818092.1	MbtH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-114)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 11.3033

>T108536 WP_190818084.1 NZ_CP031142:5197558-5197905 [Saccharopolyspora pogona]
MRRGDLYWVDFEPIRGSEANKTRPAVVISNDAANRGAQRAGRGVITVVPVTSNIDHVHPFQVLLRAAECGLPNDSKAQAE
QVRAVAVNRIGNRIGTLPQNTLAKLESALRLHLRL

Nucleotide

• Length: 348 bp

>T108536 NZ_CP031142:5197558-5197905 [Saccharopolyspora pogona]
ATGCGCCGCGGTGACCTCTACTGGGTCGACTTCGAACCCATCCGCGGCTCGGAAGCCAACAAGACCCGCCCCGCGGTCGT
CATCAGCAACGACGCCGCGAACCGCGGCGCCCAGCGCGCCGGGCGAGGAGTGATCACCGTCGTGCCGGTGACCTCCAACA
TCGACCACGTGCACCCGTTCCAGGTGCTCCTGCGGGCCGCCGAATGCGGCCTGCCCAACGACTCGAAGGCCCAGGCCGAG
CAGGTCCGGGCGGTCGCGGTCAACCGCATCGGCAACCGCATCGGAACCCTGCCGCAGAACACGCTCGCCAAGCTCGAGAG
CGCCCTCCGACTCCACCTCCGCCTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8179.89 Da
• Isoelectric Point: 3.7803

>AT108536 WP_190818082.1 NZ_CP031142:5197341-5197571 [Saccharopolyspora pogona]
MKVSVSLTEEDVTFVDEYAQQIGAPSRSAVIHQAINLLRNAGLEDAYASAWEEWDGSADAQLWDATAADGIADAPR

Nucleotide

• Length: 231 bp

>AT108536 NZ_CP031142:5197341-5197571 [Saccharopolyspora pogona]
ATGAAAGTGAGTGTGAGCCTGACCGAGGAAGACGTCACCTTCGTCGACGAGTACGCCCAGCAGATCGGCGCGCCATCGCG
GTCGGCAGTGATCCATCAGGCGATCAACCTGCTGCGCAACGCCGGCCTGGAAGACGCCTACGCCAGCGCCTGGGAGGAGT
GGGACGGCAGCGCCGACGCGCAACTGTGGGACGCGACTGCCGCCGACGGAATCGCCGATGCGCCGCGGTGA