Detailed information of TA system TA108525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2109765..2110294
• Replicon: chromosome
• Accession: NZ_CP031131
• Organism: Staphylococcus aureus strain E16SA093

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DU470_RS10925
• Protein ID: WP_000621175.1
• Coordinates: 2109765..2110127 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: DU470_RS10930
• Protein ID: WP_000948331.1
• Coordinates: 2110124..2110294 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DU470_RS10900	2106744..2107514	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
DU470_RS10905	2107489..2107968	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
DU470_RS10910	2107970..2108296	-	327	WP_001052491.1	anti-sigma factor antagonist	-
DU470_RS10915	2108415..2109416	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
DU470_RS10925	2109765..2110127	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DU470_RS10930	2110124..2110294	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
DU470_RS10935	2110379..2111527	-	1149	WP_001281142.1	alanine racemase	-
DU470_RS10940	2111593..2111952	-	360	WP_000581197.1	holo-ACP synthase	-
DU470_RS10945	2111956..2112447	-	492	WP_001205910.1	PH domain-containing protein	-
DU470_RS10950	2112434..2114017	-	1584	WP_001294626.1	PH domain-containing protein	-
DU470_RS10955	2114010..2114489	-	480	WP_001287090.1	hypothetical protein	-
DU470_RS10960	2114698..2115258	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T108525 WP_000621175.1 NZ_CP031131:c2110127-2109765 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T108525 NZ_CP031131:c2110127-2109765 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT108525 WP_000948331.1 NZ_CP031131:c2110294-2110124 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT108525 NZ_CP031131:c2110294-2110124 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1