Detailed information of TA system TA108475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 22519..23041
• Replicon: plasmid pAMSC5
• Accession: NZ_CP031110
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: DUT84_RS27455
• Protein ID: WP_000220560.1
• Coordinates: 22760..23041 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: DUT84_RS27450
• Protein ID: WP_000121743.1
• Coordinates: 22519..22770 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS27415	17597..18259	+	663	WP_000516402.1	peptidyl-arginine deiminase	-
DUT84_RS27420	18359..18640	+	282	WP_001185482.1	hypothetical protein	-
DUT84_RS27425	18665..19642	+	978	WP_001579336.1	hypothetical protein	-
DUT84_RS27430	19639..20064	-	426	WP_015058974.1	TaxD	-
DUT84_RS27435	20132..20578	-	447	WP_001074386.1	hypothetical protein	-
DUT84_RS27440	20603..21457	-	855	WP_001239324.1	replication initiation protein	-
DUT84_RS27450	22519..22770	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
DUT84_RS27455	22760..23041	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DUT84_RS27460	23187..23510	+	324	WP_001181903.1	hypothetical protein	-
DUT84_RS27465	23555..23800	+	246	WP_000356546.1	hypothetical protein	-
DUT84_RS27470	23790..24005	+	216	WP_001180116.1	hypothetical protein	-
DUT84_RS27475	24098..24427	+	330	WP_000866648.1	hypothetical protein	-
DUT84_RS27480	24470..24649	+	180	WP_000439078.1	hypothetical protein	-
DUT84_RS28875	24719..24868	+	150	WP_000003880.1	hypothetical protein	-
DUT84_RS27490	24881..25153	+	273	WP_000160399.1	hypothetical protein	-
DUT84_RS28880	25410..25484	+	75	Protein_34	DNA distortion polypeptide 3	-
DUT84_RS27500	25479..26024	+	546	WP_000757693.1	hypothetical protein	-
DUT84_RS27505	26027..27193	+	1167	WP_001576909.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qacE / aadA5 / dfrA17 / blaTEM-1B	-	1..50124	50124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T108475 WP_000220560.1 NZ_CP031110:22760-23041 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T108475 NZ_CP031110:22760-23041 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT108475 WP_000121743.1 NZ_CP031110:22519-22770 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT108475 NZ_CP031110:22519-22770 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301