Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 83645..84170 Replicon plasmid pAMSC2
Accession NZ_CP031107
Organism Escherichia coli strain AMSCJX02

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DUT84_RS26090 Protein ID WP_001159871.1
Coordinates 83645..83950 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag DUT84_RS26095 Protein ID WP_000813630.1
Coordinates 83952..84170 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DUT84_RS26060 78853..79065 - 213 WP_001523378.1 hypothetical protein -
DUT84_RS26065 79794..80051 + 258 WP_015387373.1 hypothetical protein -
DUT84_RS26070 80147..80593 - 447 WP_053270998.1 hypothetical protein -
DUT84_RS26075 80758..81986 + 1229 WP_148718718.1 IS3-like element IS2 family transposase -
DUT84_RS26080 81989..82162 - 174 Protein_92 RepB family plasmid replication initiator protein -
DUT84_RS26085 82862..83644 - 783 WP_001513526.1 site-specific integrase -
DUT84_RS26090 83645..83950 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DUT84_RS26095 83952..84170 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DUT84_RS26110 84726..85415 + 690 WP_001513525.1 helix-turn-helix domain-containing protein -
DUT84_RS26115 85447..86136 - 690 WP_001513524.1 RES family NAD+ phosphorylase -
DUT84_RS26125 86555..86785 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
DUT84_RS26130 86782..87198 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat 1..203856 203856


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T108471 WP_001159871.1 NZ_CP031107:c83950-83645 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T108471 NZ_CP031107:c83950-83645 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT108471 WP_000813630.1 NZ_CP031107:c84170-83952 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT108471 NZ_CP031107:c84170-83952 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References