Detailed information of TA system TA108461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 14223..14758
• Replicon: plasmid pAMSC2
• Accession: NZ_CP031107
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: DUT84_RS25635
• Protein ID: WP_000222760.1
• Coordinates: 14223..14510 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: DUT84_RS25640
• Protein ID: WP_001132900.1
• Coordinates: 14507..14758 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS25595	9806..11027	-	1222	Protein_7	ISL3 family transposase	-
DUT84_RS25605	11127..11491	+	365	Protein_8	IS1 family transposase	-
DUT84_RS25610	11546..12328	-	783	WP_001442137.1	IS21-like element IS100kyp family helper ATPase IstB	-
DUT84_RS25615	12325..13347	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
DUT84_RS25625	13436..13783	+	348	Protein_11	IS1-like element IS1A family transposase	-
DUT84_RS25635	14223..14510	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DUT84_RS25640	14507..14758	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DUT84_RS25655	15725..16582	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
DUT84_RS25660	16575..17057	-	483	WP_001273588.1	hypothetical protein	-
DUT84_RS25665	17050..17124	-	75	WP_001442103.1	RepA leader peptide Tap	-
DUT84_RS25675	17356..17613	-	258	WP_000083845.1	replication regulatory protein RepA	-
DUT84_RS25680	17897..18103	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
-	18090..18151	+	62	NuclAT_1	-	-
-	18090..18151	+	62	NuclAT_1	-	-
-	18090..18151	+	62	NuclAT_1	-	-
-	18090..18151	+	62	NuclAT_1	-	-
DUT84_RS28800	18407..18481	-	75	Protein_19	endonuclease	-
DUT84_RS25690	18727..18939	-	213	WP_001299730.1	ANR family transcriptional regulator	-
DUT84_RS25695	19075..19635	-	561	WP_032072881.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..203856	203856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T108461 WP_000222760.1 NZ_CP031107:c14510-14223 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T108461 NZ_CP031107:c14510-14223 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT108461 WP_001132900.1 NZ_CP031107:c14758-14507 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT108461 NZ_CP031107:c14758-14507 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2