Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4587156..4587794 Replicon chromosome
Accession NZ_CP031105
Organism Escherichia coli strain AMSCJX02

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag DUT84_RS23680 Protein ID WP_000813794.1
Coordinates 4587156..4587332 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag DUT84_RS23685 Protein ID WP_001270286.1
Coordinates 4587378..4587794 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DUT84_RS23660 4582775..4583989 - 1215 WP_001307190.1 BenE family transporter YdcO -
DUT84_RS23665 4584042..4584578 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
DUT84_RS23670 4584651..4586612 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
DUT84_RS23675 4586704..4586934 - 231 WP_000494239.1 YncJ family protein -
DUT84_RS23680 4587156..4587332 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
DUT84_RS23685 4587378..4587794 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
DUT84_RS23690 4587873..4589279 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
DUT84_RS23695 4589524..4590669 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
DUT84_RS23700 4590687..4591700 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
DUT84_RS23705 4591701..4592642 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T108458 WP_000813794.1 NZ_CP031105:4587156-4587332 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T108458 NZ_CP031105:4587156-4587332 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT108458 WP_001270286.1 NZ_CP031105:4587378-4587794 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT108458 NZ_CP031105:4587378-4587794 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGTGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References