Detailed information of TA system TA108452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3543856..3544693
• Replicon: chromosome
• Accession: NZ_CP031105
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: DUT84_RS18225
• Protein ID: WP_096262872.1
• Coordinates: 3543856..3544398 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: DUT84_RS18230
• Protein ID: WP_001297137.1
• Coordinates: 3544382..3544693 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS18205	3539395..3540306	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
DUT84_RS18210	3540474..3540965	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
DUT84_RS18215	3541093..3542457	-	1365	WP_001000978.1	MFS transporter	-
DUT84_RS18220	3542865..3543800	+	936	WP_001297127.1	sel1 repeat family protein	-
DUT84_RS18225	3543856..3544398	-	543	WP_096262872.1	GNAT family N-acetyltransferase	Toxin
DUT84_RS18230	3544382..3544693	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
DUT84_RS18235	3544878..3545768	-	891	WP_000971336.1	heme o synthase	-
DUT84_RS18240	3545780..3546109	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
DUT84_RS18245	3546109..3546723	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
DUT84_RS18250	3546713..3548704	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
DUT84_RS18255	3548726..3549673	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19804.03 Da
• Isoelectric Point: 8.6178

>T108452 WP_096262872.1 NZ_CP031105:c3544398-3543856 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSNITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T108452 NZ_CP031105:c3544398-3543856 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTAATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT108452 WP_001297137.1 NZ_CP031105:c3544693-3544382 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT108452 NZ_CP031105:c3544693-3544382 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3