Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 2037335..2037966 Replicon chromosome
Accession NZ_CP031105
Organism Escherichia coli strain AMSCJX02

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag DUT84_RS10690 Protein ID WP_001259385.1
Coordinates 2037691..2037966 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag DUT84_RS10685 Protein ID WP_000593554.1
Coordinates 2037335..2037694 (-) Length 120 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DUT84_RS10655 2032447..2032920 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
DUT84_RS10660 2032917..2033198 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
DUT84_RS10665 2033275..2034633 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
DUT84_RS10670 2034626..2036134 + 1509 WP_001298718.1 carbohydrate kinase -
DUT84_RS10675 2036124..2036393 + 270 WP_000933060.1 HPr family phosphocarrier protein -
DUT84_RS10680 2036425..2037285 + 861 WP_001131782.1 class II aldolase -
DUT84_RS10685 2037335..2037694 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DUT84_RS10690 2037691..2037966 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
DUT84_RS10695 2038039..2039163 - 1125 WP_001314210.1 ABC transporter permease -
DUT84_RS10700 2039163..2041898 - 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
DUT84_RS10705 2041895..2042962 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(30-80)

Antitoxin

(60-112)


Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T108448 WP_001259385.1 NZ_CP031105:c2037966-2037691 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T108448 NZ_CP031105:c2037966-2037691 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT108448 WP_000593554.1 NZ_CP031105:c2037694-2037335 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT108448 NZ_CP031105:c2037694-2037335 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8


Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References