Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1009242..1009867 Replicon chromosome
Accession NZ_CP031105
Organism Escherichia coli strain AMSCJX02

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag DUT84_RS05330 Protein ID WP_000911329.1
Coordinates 1009242..1009640 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag DUT84_RS05335 Protein ID WP_000450524.1
Coordinates 1009640..1009867 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DUT84_RS05310 1005120..1005320 + 201 WP_000383836.1 YpfN family protein -
DUT84_RS05315 1005430..1006128 - 699 WP_000679812.1 esterase -
DUT84_RS05320 1006202..1008217 - 2016 WP_000829393.1 tRNA cytosine(34) acetyltransferase TmcA -
DUT84_RS05325 1008232..1009095 - 864 WP_112021547.1 neutral zinc metallopeptidase -
DUT84_RS05330 1009242..1009640 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
DUT84_RS05335 1009640..1009867 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
DUT84_RS05340 1010022..1010735 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
DUT84_RS05345 1010948..1011982 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
DUT84_RS05350 1011999..1012877 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
DUT84_RS05355 1013023..1013595 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
DUT84_RS05360 1013595..1014065 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T108443 WP_000911329.1 NZ_CP031105:c1009640-1009242 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T108443 NZ_CP031105:c1009640-1009242 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT108443 WP_000450524.1 NZ_CP031105:c1009867-1009640 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT108443 NZ_CP031105:c1009867-1009640 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References