Detailed information of TA system TA108428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 3067447..3068241
• Replicon: chromosome
• Accession: NZ_CP031104
• Organism: Leclercia sp. W6

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: DVA43_RS14985
• Protein ID: WP_103823102.1
• Coordinates: 3067720..3068241 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: DVA43_RS14980
• Protein ID: WP_103823103.1
• Coordinates: 3067447..3067716 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVA43_RS14960	3064020..3065099	-	1080	WP_103823107.1	DUF2955 domain-containing protein	-
DVA43_RS14965	3065102..3066169	-	1068	WP_103823106.1	HlyD family secretion protein	-
DVA43_RS14970	3066169..3066636	-	468	WP_114315995.1	MarR family transcriptional regulator	-
DVA43_RS14975	3066733..3067122	-	390	WP_114315996.1	hypothetical protein	-
DVA43_RS14980	3067447..3067716	+	270	WP_103823103.1	DUF1778 domain-containing protein	Antitoxin
DVA43_RS14985	3067720..3068241	+	522	WP_103823102.1	GNAT family N-acetyltransferase	Toxin
DVA43_RS14990	3068294..3068989	-	696	WP_114315997.1	winged helix-turn-helix domain-containing protein	-
DVA43_RS14995	3068964..3069359	-	396	WP_114315998.1	amino acid-binding protein	-
DVA43_RS15000	3069352..3069675	-	324	WP_103823099.1	antibiotic biosynthesis monooxygenase	-
DVA43_RS15005	3069912..3071327	-	1416	WP_114315999.1	aldehyde dehydrogenase family protein	-
DVA43_RS15010	3071428..3072252	+	825	WP_168194661.1	LysR family transcriptional regulator	-
DVA43_RS15015	3072317..3072796	-	480	WP_114316000.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19442.43 Da
• Isoelectric Point: 9.5128

>T108428 WP_103823102.1 NZ_CP031104:3067720-3068241 [Leclercia sp. W6]
VTKLTIRMFSHEARYDLTDFNCGDSTLNRFLFDHLARQHSGRLLRAYLLVTDAPEPKVLGYYTLSGSCFEKETLPSKTQQ
RKVPYVNVPSVTLGRLAIHQDFQGQGWGMTLVTHAMKVVYLASQAVGIHGMFVDALNDKAKAFYLKLGFISLTGSNANSL
FYPTKSIEKLFVE

Nucleotide

• Length: 522 bp

>T108428 NZ_CP031104:3067720-3068241 [Leclercia sp. W6]
GTGACTAAACTGACGATAAGGATGTTTTCGCATGAGGCACGCTATGATTTAACAGACTTCAACTGCGGGGATAGCACCCT
GAACCGCTTTCTGTTCGATCACCTTGCACGCCAACACAGCGGGCGCCTGCTACGCGCCTATCTTCTTGTCACCGATGCCC
CTGAGCCAAAAGTGCTGGGTTACTATACGTTGTCCGGCAGTTGCTTTGAGAAAGAGACACTGCCCTCAAAAACGCAGCAG
CGCAAAGTACCGTACGTCAATGTGCCCAGCGTCACGTTAGGACGTCTCGCTATACATCAGGATTTTCAGGGCCAGGGCTG
GGGGATGACGCTGGTGACCCATGCCATGAAAGTGGTCTACCTGGCCTCACAGGCCGTTGGCATTCACGGTATGTTTGTGG
ATGCACTCAACGATAAAGCGAAAGCGTTTTATCTCAAGCTCGGCTTTATCTCGCTAACCGGCAGCAATGCCAACTCTCTT
TTTTATCCTACAAAATCAATCGAGAAGCTGTTTGTTGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10334.68 Da
• Isoelectric Point: 4.8839

>AT108428 WP_103823103.1 NZ_CP031104:3067447-3067716 [Leclercia sp. W6]
MSALKKQRIDLRLTEEDKDLIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLILNENSWNRVMDALDNPPEPNDQLKR
AAKRLQNME

Nucleotide

• Length: 270 bp

>AT108428 NZ_CP031104:3067447-3067716 [Leclercia sp. W6]
ATGTCAGCACTGAAAAAACAGCGCATCGACCTCAGGTTAACGGAAGAAGACAAGGATCTGATCGAAGAGGCTGCGGCGAT
GACTAACCAGACAATTACCCAGTTTATGGTTAACAGTGCCTCTGAACGTGCTGCAGAAGTCATTGAGCAGCATCGTCGTC
TCATCCTCAATGAAAATTCGTGGAACCGGGTGATGGATGCGCTCGACAATCCGCCAGAGCCGAACGACCAGCTGAAACGC
GCAGCGAAACGCTTACAAAACATGGAGTAA