Detailed information of TA system TA108415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 86803..87446
• Replicon: plasmid pW17-2
• Accession: NZ_CP031103
• Organism: Leclercia sp. W17

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: DVA44_RS25445
• Protein ID: WP_114388530.1
• Coordinates: 86803..87219 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A345CHX5
• Locus tag: DVA44_RS25450
• Protein ID: WP_039032651.1
• Coordinates: 87216..87446 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVA44_RS25425	82169..82519	+	351	WP_114388527.1	DUF305 domain-containing protein	-
DVA44_RS25430	82705..83613	-	909	WP_114388538.1	HNH endonuclease	-
DVA44_RS25435	84160..85182	-	1023	WP_114388528.1	DNA helicase UvrD	-
DVA44_RS25440	85167..86729	-	1563	WP_114388529.1	AAA family ATPase	-
DVA44_RS25445	86803..87219	-	417	WP_114388530.1	type II toxin-antitoxin system VapC family toxin	Toxin
DVA44_RS25450	87216..87446	-	231	WP_039032651.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DVA44_RS25455	87974..88288	+	315	WP_114388531.1	hypothetical protein	-
DVA44_RS25460	88399..88691	-	293	Protein_96	hypothetical protein	-
DVA44_RS25800	90155..90328	-	174	WP_168194531.1	hypothetical protein	-
DVA44_RS25470	90419..90991	-	573	WP_104677981.1	hypothetical protein	-
DVA44_RS25475	91078..92199	-	1122	WP_114388533.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92616	92616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15153.59 Da
• Isoelectric Point: 7.1361

>T108415 WP_114388530.1 NZ_CP031103:c87219-86803 [Leclercia sp. W17]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRSQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T108415 NZ_CP031103:c87219-86803 [Leclercia sp. W17]
GTGAACAAGATTTATATGCTCGACACGAACATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCAGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCCGAGATGCGCTTCGGCGCCACCGGGC
CGAAGGCCTCACCGCGGCATATCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTGGATGCGACTACAGAAATCAAGGTGGCGCTGCGCCTCGCGGGTACACCGATCGGGCCGAACGACACGGCGATCGC
CGGGCACGCCATTGCCGCCGGCGCGGTGCTGGTGACGAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8737.83 Da
• Isoelectric Point: 4.3654

>AT108415 WP_039032651.1 NZ_CP031103:c87446-87216 [Leclercia sp. W17]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT108415 NZ_CP031103:c87446-87216 [Leclercia sp. W17]
ATGAGAACAGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
AAAAAGCAGATCCGGACTTTATGGCGGAGCGTGAGGACGTTGTCACCGACGAAGGACGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A345CHX5