Detailed information of TA system TA108394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 5332037..5332629
• Replicon: chromosome
• Accession: NZ_CP031087
• Organism: Actinokineospora sp. UTMC 2448

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Actkin_RS24300
• Protein ID: WP_258904386.1
• Coordinates: 5332037..5332390 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Actkin_RS24305
• Protein ID: WP_258904387.1
• Coordinates: 5332384..5332629 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Actkin_RS24275 (Actkin_04846)	5327677..5328801	-	1125	WP_258904384.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
Actkin_RS24280 (Actkin_04847)	5328834..5330051	-	1218	WP_189214464.1	site-2 protease family protein	-
Actkin_RS24285 (Actkin_04848)	5330048..5331214	-	1167	WP_189214465.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
Actkin_RS24290 (Actkin_04849)	5331392..5331658	+	267	WP_189214466.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
Actkin_RS24295 (Actkin_04850)	5331757..5332032	+	276	WP_229787852.1	DUF2631 domain-containing protein	-
Actkin_RS24300 (Actkin_04851)	5332037..5332390	-	354	WP_258904386.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Actkin_RS24305 (Actkin_04852)	5332384..5332629	-	246	WP_258904387.1	ribbon-helix-helix domain-containing protein	Antitoxin
Actkin_RS24310 (Actkin_04853)	5332661..5333764	-	1104	WP_189214470.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
Actkin_RS24315 (Actkin_04854)	5333875..5334519	+	645	WP_189214471.1	PadR family transcriptional regulator	-
Actkin_RS24320 (Actkin_04855)	5334516..5335253	-	738	WP_258907983.1	class I SAM-dependent methyltransferase	-
Actkin_RS24325 (Actkin_04856)	5335333..5336178	+	846	WP_258904388.1	VOC family protein	-
Actkin_RS24330 (Actkin_04857)	5336175..5337011	-	837	WP_258904389.1	phosphatidate cytidylyltransferase	-
Actkin_RS24335 (Actkin_04858)	5337037..5337594	-	558	WP_189214474.1	ribosome recycling factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-114)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12355.22 Da
• Isoelectric Point: 10.9644

>T108394 WP_258904386.1 NZ_CP031087:c5332390-5332037 [Actinokineospora sp. UTMC 2448]
VVIRRGGVHWADLGDAQGSRPAKRRPVLVVQADPYNRSRLATVLVAVITSNTKLATAPGNVFLSAAATGLPRDSVVNVTA
VVTLDKDDLSDAVATLPPSLMREVDAGLRRVMGLRSD

Nucleotide

• Length: 354 bp

>T108394 NZ_CP031087:c5332390-5332037 [Actinokineospora sp. UTMC 2448]
GTGGTGATCAGACGAGGCGGCGTCCACTGGGCCGACCTCGGTGACGCCCAGGGGTCCCGGCCAGCCAAGCGGCGCCCGGT
GCTGGTCGTCCAGGCCGACCCCTACAACCGCAGCAGACTGGCGACCGTCCTGGTCGCCGTGATCACGTCCAATACGAAGC
TGGCGACCGCGCCGGGGAACGTCTTCCTCTCCGCCGCGGCGACGGGTCTGCCCCGTGACTCGGTGGTCAACGTGACCGCG
GTGGTCACCCTCGACAAGGACGACCTGAGCGATGCCGTGGCCACGCTTCCGCCCAGCCTGATGCGCGAGGTCGACGCGGG
ACTGCGCCGCGTCATGGGCCTGCGGTCGGACTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8793.61 Da
• Isoelectric Point: 4.0987

>AT108394 WP_258904387.1 NZ_CP031087:c5332629-5332384 [Actinokineospora sp. UTMC 2448]
VKTAISVPDDTFAEVEDKVRNLGLTRSEFYVRAARHYLAHLEEQSLTDEIDAALDLAGDDDSGAVAAAAGRRALAMSDDE
W

Nucleotide

• Length: 246 bp

>AT108394 NZ_CP031087:c5332629-5332384 [Actinokineospora sp. UTMC 2448]
GTGAAGACCGCGATTTCCGTGCCCGACGACACGTTCGCCGAGGTGGAGGACAAGGTGCGCAACCTGGGCCTCACCCGCTC
GGAGTTCTACGTGCGCGCGGCCCGGCACTACCTGGCCCATCTGGAAGAACAGTCCCTGACCGACGAGATCGACGCCGCGC
TCGACCTCGCGGGCGACGACGACTCCGGCGCGGTGGCGGCCGCGGCGGGCAGGCGCGCGCTGGCCATGTCGGACGATGAG
TGGTGA