Detailed information of TA system TA108387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2423789..2424767
• Replicon: chromosome
• Accession: NZ_CP031071
• Organism: Bacillus mycoides strain BPN401

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: R8N1U0
• Locus tag: DN409_RS12455
• Protein ID: WP_002032139.1
• Coordinates: 2423789..2424529 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: J8YSJ3
• Locus tag: DN409_RS12460
• Protein ID: WP_002069312.1
• Coordinates: 2424642..2424767 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN409_RS12425	2419759..2420469	+	711	WP_061689428.1	class I SAM-dependent methyltransferase	-
DN409_RS12430	2420588..2421001	+	414	WP_002012925.1	VOC family protein	-
DN409_RS12435	2421178..2421810	-	633	WP_002017047.1	glutamine amidotransferase	-
DN409_RS12440	2422084..2422560	+	477	WP_061689429.1	MarR family winged helix-turn-helix transcriptional regulator	-
DN409_RS12445	2422714..2423451	+	738	WP_002032137.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
DN409_RS12455	2423789..2424529	+	741	WP_002032139.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DN409_RS12460	2424642..2424767	+	126	WP_002069312.1	hypothetical protein	Antitoxin
DN409_RS12465	2424843..2425019	+	177	WP_002141782.1	hypothetical protein	-
DN409_RS12470	2425063..2425338	-	276	WP_002141783.1	hypothetical protein	-
DN409_RS12475	2425365..2425895	-	531	WP_040963068.1	hypothetical protein	-
DN409_RS30880	2426217..2426393	+	177	WP_002141786.1	hypothetical protein	-
DN409_RS12480	2426469..2426858	-	390	WP_061689430.1	YxeA family protein	-
DN409_RS12485	2427110..2428579	+	1470	WP_149190503.1	aminoacyl-histidine dipeptidase	-
DN409_RS30885	2428939..2429088	+	150	WP_187770547.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28482.11 Da
• Isoelectric Point: 8.3034

>T108387 WP_002032139.1 NZ_CP031071:2423789-2424529 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFTIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T108387 NZ_CP031071:2423789-2424529 [Bacillus mycoides]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCACGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ACGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4937.36 Da
• Isoelectric Point: 3.9798

>AT108387 WP_002069312.1 NZ_CP031071:2424642-2424767 [Bacillus mycoides]
VIKDYDNDFFKENNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT108387 NZ_CP031071:2424642-2424767 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAAATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: R8N1U0

Antitoxin

• AlphaFold DB: J8YSJ3