Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245934..246576 Replicon chromosome
Accession NZ_CP031071
Organism Bacillus mycoides strain BPN401

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag DN409_RS01395 Protein ID WP_000635965.1
Coordinates 246226..246576 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag DN409_RS01390 Protein ID WP_000004570.1
Coordinates 245934..246221 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DN409_RS01365 241087..242049 + 963 WP_061689866.1 UV DNA damage repair endonuclease UvsE -
DN409_RS01370 242229..242777 - 549 WP_002139859.1 rhomboid family intramembrane serine protease -
DN409_RS01375 242869..243228 + 360 WP_002009969.1 holo-ACP synthase -
DN409_RS01380 243385..244335 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
DN409_RS01385 244453..245622 + 1170 WP_061689867.1 alanine racemase -
DN409_RS01390 245934..246221 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
DN409_RS01395 246226..246576 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DN409_RS01400 246645..248813 + 2169 WP_002029442.1 RNA-binding transcriptional accessory protein -
DN409_RS01405 248871..248987 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
DN409_RS01410 249198..249653 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T108386 WP_000635965.1 NZ_CP031071:246226-246576 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T108386 NZ_CP031071:246226-246576 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT108386 WP_000004570.1 NZ_CP031071:245934-246221 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT108386 NZ_CP031071:245934-246221 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References