Detailed information of TA system TA108385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2220985..2221963
• Replicon: chromosome
• Accession: NZ_CP031068
• Organism: Bacillus sp. JAS24-2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: DN407_RS11600
• Protein ID: WP_000624983.1
• Coordinates: 2220985..2221722 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: DN407_RS11605
• Protein ID: WP_000237818.1
• Coordinates: 2221835..2221963 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN407_RS11575	2216601..2217008	+	408	WP_000063589.1	VOC family protein	-
DN407_RS11580	2217030..2217410	+	381	Protein_2179	methyltransferase	-
DN407_RS11585	2217568..2219253	-	1686	WP_050552233.1	alpha-keto acid decarboxylase family protein	-
DN407_RS11590	2219361..2219843	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
DN407_RS11595	2220010..2220747	+	738	WP_078187382.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
DN407_RS11600	2220985..2221722	+	738	WP_000624983.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DN407_RS11605	2221835..2221963	+	129	WP_000237818.1	hypothetical protein	Antitoxin
DN407_RS11610	2222036..2222212	+	177	WP_000852629.1	stage II sporulation protein SB	-
DN407_RS11615	2222232..2222621	-	390	WP_000713865.1	YxeA family protein	-
DN407_RS11620	2222833..2224302	+	1470	WP_000287515.1	beta-Ala-His dipeptidase	-
DN407_RS11625	2224548..2225357	+	810	WP_000678509.1	C39 family peptidase	-
DN407_RS11630	2225383..2225985	+	603	WP_000517260.1	hypothetical protein	-
DN407_RS11635	2226225..2226476	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28323.03 Da
• Isoelectric Point: 8.2998

>T108385 WP_000624983.1 NZ_CP031068:2220985-2221722 [Bacillus sp. JAS24-2]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDISF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T108385 NZ_CP031068:2220985-2221722 [Bacillus sp. JAS24-2]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTTCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCAAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT108385 WP_000237818.1 NZ_CP031068:2221835-2221963 [Bacillus sp. JAS24-2]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT108385 NZ_CP031068:2221835-2221963 [Bacillus sp. JAS24-2]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5