Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239268..239910 Replicon chromosome
Accession NZ_CP031065
Organism Bacillus sp. SH8-8

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag DN405_RS01370 Protein ID WP_000635965.1
Coordinates 239560..239910 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag DN405_RS01365 Protein ID WP_000004570.1
Coordinates 239268..239555 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DN405_RS01340 234585..235547 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
DN405_RS01345 235540..236112 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
DN405_RS01350 236206..236565 + 360 WP_000583415.1 holo-ACP synthase -
DN405_RS01355 236722..237672 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
DN405_RS01360 237790..238959 + 1170 WP_016124210.1 alanine racemase -
DN405_RS01365 239268..239555 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
DN405_RS01370 239560..239910 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DN405_RS01375 239978..242146 + 2169 WP_016124211.1 RNA-binding transcriptional accessory protein -
DN405_RS01380 242204..242320 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
DN405_RS01385 242516..242974 + 459 WP_000344237.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T108382 WP_000635965.1 NZ_CP031065:239560-239910 [Bacillus sp. SH8-8]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T108382 NZ_CP031065:239560-239910 [Bacillus sp. SH8-8]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT108382 WP_000004570.1 NZ_CP031065:239268-239555 [Bacillus sp. SH8-8]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT108382 NZ_CP031065:239268-239555 [Bacillus sp. SH8-8]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References