Detailed information of TA system TA108381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2263215..2264193
• Replicon: chromosome
• Accession: NZ_CP031062
• Organism: Bacillus sp. AR4-2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: DN399_RS11755
• Protein ID: WP_149149580.1
• Coordinates: 2263215..2263952 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: DN399_RS11760
• Protein ID: WP_000237818.1
• Coordinates: 2264065..2264193 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN399_RS11735	2258867..2259639	+	773	Protein_2194	class I SAM-dependent methyltransferase	-
DN399_RS11740	2259797..2261482	-	1686	WP_149149579.1	alpha-keto acid decarboxylase family protein	-
DN399_RS11745	2261590..2262072	+	483	WP_000191886.1	MarR family winged helix-turn-helix transcriptional regulator	-
DN399_RS11750	2262239..2262976	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
DN399_RS11755	2263215..2263952	+	738	WP_149149580.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DN399_RS11760	2264065..2264193	+	129	WP_000237818.1	hypothetical protein	Antitoxin
DN399_RS11765	2264266..2264442	+	177	WP_065382523.1	stage II sporulation protein SB	-
DN399_RS11770	2264519..2264851	-	333	WP_149149581.1	YxeA family protein	-
DN399_RS11775	2265063..2266532	+	1470	WP_000287539.1	beta-Ala-His dipeptidase	-
DN399_RS11780	2266778..2267587	+	810	WP_000664544.1	C39 family peptidase	-
DN399_RS11785	2267613..2268215	+	603	WP_149149582.1	hypothetical protein	-
DN399_RS11790	2268459..2268710	-	252	WP_149149583.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28250.92 Da
• Isoelectric Point: 7.3941

>T108381 WP_149149580.1 NZ_CP031062:2263215-2263952 [Bacillus sp. AR4-2]
MISNIQIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMAIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T108381 NZ_CP031062:2263215-2263952 [Bacillus sp. AR4-2]
ATGATCTCTAACATTCAAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACTGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGCTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCTGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT108381 WP_000237818.1 NZ_CP031062:2264065-2264193 [Bacillus sp. AR4-2]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT108381 NZ_CP031062:2264065-2264193 [Bacillus sp. AR4-2]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAGTCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5