Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239273..239915 Replicon chromosome
Accession NZ_CP031062
Organism Bacillus sp. AR4-2

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag DN399_RS01370 Protein ID WP_000635965.1
Coordinates 239565..239915 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag DN399_RS01365 Protein ID WP_000004570.1
Coordinates 239273..239560 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DN399_RS01340 234590..235552 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
DN399_RS01345 235545..236117 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
DN399_RS01350 236211..236570 + 360 WP_000583415.1 holo-ACP synthase -
DN399_RS01355 236727..237677 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
DN399_RS01360 237795..238964 + 1170 WP_016124210.1 alanine racemase -
DN399_RS01365 239273..239560 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
DN399_RS01370 239565..239915 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DN399_RS01375 239983..242151 + 2169 WP_016124211.1 RNA-binding transcriptional accessory protein -
DN399_RS01380 242209..242325 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
DN399_RS01385 242521..242979 + 459 WP_000344237.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T108380 WP_000635965.1 NZ_CP031062:239565-239915 [Bacillus sp. AR4-2]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T108380 NZ_CP031062:239565-239915 [Bacillus sp. AR4-2]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT108380 WP_000004570.1 NZ_CP031062:239273-239560 [Bacillus sp. AR4-2]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT108380 NZ_CP031062:239273-239560 [Bacillus sp. AR4-2]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References