Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5521551..5522176 Replicon chromosome
Accession NZ_CP031061
Organism Klebsiella variicola strain GN02

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag DUZ89_RS26605 Protein ID WP_008807903.1
Coordinates 5521551..5521934 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DUZ89_RS26610 Protein ID WP_004150355.1
Coordinates 5521934..5522176 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DUZ89_RS26590 5518917..5519819 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DUZ89_RS26595 5519816..5520451 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
DUZ89_RS26600 5520448..5521377 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DUZ89_RS26605 5521551..5521934 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
DUZ89_RS26610 5521934..5522176 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
DUZ89_RS26615 5522381..5523298 + 918 WP_023296845.1 alpha/beta hydrolase -
DUZ89_RS26620 5523313..5524254 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
DUZ89_RS26625 5524299..5524736 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
DUZ89_RS26630 5524733..5525593 - 861 WP_008807907.1 virulence factor BrkB family protein -
DUZ89_RS26635 5525587..5526186 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T108379 WP_008807903.1 NZ_CP031061:c5521934-5521551 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T108379 NZ_CP031061:c5521934-5521551 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT108379 WP_004150355.1 NZ_CP031061:c5522176-5521934 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT108379 NZ_CP031061:c5522176-5521934 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References