Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4224506..4225125 Replicon chromosome
Accession NZ_CP031061
Organism Klebsiella variicola strain GN02

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag DUZ89_RS20455 Protein ID WP_002892050.1
Coordinates 4224907..4225125 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag DUZ89_RS20450 Protein ID WP_008805436.1
Coordinates 4224506..4224880 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DUZ89_RS20440 4219661..4220854 + 1194 WP_043875731.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DUZ89_RS20445 4220877..4224023 + 3147 WP_012968813.1 multidrug efflux RND transporter permease subunit -
DUZ89_RS20450 4224506..4224880 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
DUZ89_RS20455 4224907..4225125 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
DUZ89_RS20460 4225284..4225850 + 567 WP_016160393.1 maltose O-acetyltransferase -
DUZ89_RS20465 4225822..4225950 - 129 Protein_4016 hypothetical protein -
DUZ89_RS20470 4225987..4226457 + 471 WP_008805434.1 YlaC family protein -
DUZ89_RS20475 4226426..4227883 - 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
DUZ89_RS20480 4227984..4228682 + 699 WP_016160391.1 GNAT family N-acetyltransferase -
DUZ89_RS20485 4228679..4228819 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
DUZ89_RS20490 4228819..4229082 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T108377 WP_002892050.1 NZ_CP031061:4224907-4225125 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T108377 NZ_CP031061:4224907-4225125 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT108377 WP_008805436.1 NZ_CP031061:4224506-4224880 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT108377 NZ_CP031061:4224506-4224880 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References