Detailed information of TA system TA108367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1831221..1831811
• Replicon: chromosome
• Accession: NZ_CP031061
• Organism: Klebsiella variicola strain GN02

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DUZ89_RS08800
• Protein ID: WP_049010777.1
• Coordinates: 1831479..1831811 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A1F2LZQ3
• Locus tag: DUZ89_RS08795
• Protein ID: WP_012541132.1
• Coordinates: 1831221..1831478 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUZ89_RS08790	1828710..1830659	-	1950	WP_185185867.1	hypothetical protein	-
DUZ89_RS08795	1831221..1831478	+	258	WP_012541132.1	antitoxin	Antitoxin
DUZ89_RS08800	1831479..1831811	+	333	WP_049010777.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DUZ89_RS08810	1832134..1833570	+	1437	WP_016161429.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
DUZ89_RS08820	1833943..1835397	-	1455	WP_008804170.1	AMP nucleosidase	-
DUZ89_RS08825	1835528..1835773	-	246	WP_008804171.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1814525..1831811	17286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11895.77 Da
• Isoelectric Point: 10.1839

>T108367 WP_049010777.1 NZ_CP031061:1831479-1831811 [Klebsiella variicola]
MDRGEIWLVSLDPIVGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T108367 NZ_CP031061:1831479-1831811 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGTTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGTAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9420.91 Da
• Isoelectric Point: 4.4915

>AT108367 WP_012541132.1 NZ_CP031061:1831221-1831478 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT108367 NZ_CP031061:1831221-1831478 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZQ3