Detailed information of TA system TA108364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 327377..327963
• Replicon: chromosome
• Accession: NZ_CP031061
• Organism: Klebsiella variicola strain GN02

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DUZ89_RS01495
• Protein ID: WP_012967118.1
• Coordinates: 327595..327963 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: DUZ89_RS01490
• Protein ID: WP_004174006.1
• Coordinates: 327377..327598 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUZ89_RS01470	323526..324452	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
DUZ89_RS01475	324449..325726	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
DUZ89_RS01480	325723..326490	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
DUZ89_RS01485	326492..327205	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
DUZ89_RS01490	327377..327598	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DUZ89_RS01495	327595..327963	+	369	WP_012967118.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DUZ89_RS01500	328255..329571	+	1317	WP_008806974.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
DUZ89_RS01505	329678..330565	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
DUZ89_RS01510	330562..331407	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
DUZ89_RS01515	331409..332479	+	1071	WP_049152992.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	324449..333216	8767

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (26-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13584.98 Da
• Isoelectric Point: 8.6410

>T108364 WP_012967118.1 NZ_CP031061:327595-327963 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGMTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T108364 NZ_CP031061:327595-327963 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTATGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCCGACTTCGTCGGCATGACCGTCGAGGCTGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT108364 WP_004174006.1 NZ_CP031061:327377-327598 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT108364 NZ_CP031061:327377-327598 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7