Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 286507..287138 Replicon chromosome
Accession NZ_CP031061
Organism Klebsiella variicola strain GN02

Toxin (Protein)


Gene name hicA Uniprot ID A0A2V3KJT3
Locus tag DUZ89_RS01285 Protein ID WP_044650325.1
Coordinates 286507..286782 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag DUZ89_RS01290 Protein ID WP_004181489.1
Coordinates 286779..287138 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DUZ89_RS01265 281743..282078 + 336 WP_002921203.1 universal stress protein UspB -
DUZ89_RS01270 282138..283634 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
DUZ89_RS01275 283864..285057 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
DUZ89_RS01280 285097..286110 - 1014 WP_114500713.1 magnesium transporter -
DUZ89_RS01285 286507..286782 + 276 WP_044650325.1 type II toxin-antitoxin system HicA family toxin Toxin
DUZ89_RS01290 286779..287138 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DUZ89_RS01295 287166..287567 - 402 WP_023323361.1 nickel-responsive transcriptional regulator NikR -
DUZ89_RS01300 287555..288346 - 792 WP_008806945.1 nickel import ATP-binding protein NikE -
DUZ89_RS01305 288343..289107 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
DUZ89_RS01310 289107..289940 - 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
DUZ89_RS01315 289937..290881 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10279.91 Da        Isoelectric Point: 10.3165

>T108363 WP_044650325.1 NZ_CP031061:286507-286782 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T108363 NZ_CP031061:286507-286782 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT108363 WP_004181489.1 NZ_CP031061:286779-287138 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT108363 NZ_CP031061:286779-287138 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAGTATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V3KJT3

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References