Detailed information of TA system TA108354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 541526..542087
• Replicon: chromosome
• Accession: NZ_CP031057
• Organism: Acidipropionibacterium acidipropionici strain WGS7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K7S927
• Locus tag: DUY81_RS02320
• Protein ID: WP_015071954.1
• Coordinates: 541740..542087 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K7S105
• Locus tag: DUY81_RS02315
• Protein ID: WP_015071955.1
• Coordinates: 541526..541753 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUY81_RS02305	538544..538948	+	405	WP_015071959.1	nitrite reductase small subunit NirD	-
DUY81_RS02310	539058..541199	-	2142	WP_062819700.1	NAD(+) synthase	-
DUY81_RS02315	541526..541753	+	228	WP_015071955.1	ribbon-helix-helix domain-containing protein	Antitoxin
DUY81_RS02320	541740..542087	+	348	WP_015071954.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DUY81_RS02325	542240..543199	-	960	WP_062819699.1	ribokinase	-
DUY81_RS02330	543365..544909	+	1545	WP_062819698.1	glycoside hydrolase family 32 protein	-
DUY81_RS02340	545439..545816	+	378	WP_082816119.1	L,D-transpeptidase	-
DUY81_RS02345	545947..546921	-	975	WP_198401490.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-35)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12548.53 Da
• Isoelectric Point: 11.4911

>T108354 WP_015071954.1 NZ_CP031057:541740-542087 [Acidipropionibacterium acidipropionici]
MIRGEVRLVDLDPARPGEVNKRRPAVLVSNDTANRTAARLGRGVVTVVPVTSNVTRVFPFQVLLQADETGLRRDSKAQAE
QVRSVSVERIGPVLGMVPADLMSRIDDALRIHLGL

Nucleotide

• Length: 348 bp

>T108354 NZ_CP031057:541740-542087 [Acidipropionibacterium acidipropionici]
ATGATCCGCGGTGAGGTCCGGCTGGTCGATCTGGATCCGGCGCGCCCCGGCGAGGTGAACAAGCGACGTCCCGCCGTCCT
CGTGAGCAATGACACGGCGAACCGGACGGCCGCACGGCTGGGGCGGGGAGTGGTGACGGTGGTGCCGGTCACCAGCAACG
TCACCCGGGTCTTCCCCTTCCAGGTCCTGCTTCAGGCGGACGAGACGGGGCTGCGGCGCGATTCCAAGGCCCAGGCCGAG
CAGGTCCGCTCGGTGTCGGTGGAACGGATCGGCCCGGTGCTCGGGATGGTGCCGGCTGATCTCATGAGCCGCATCGATGA
CGCGCTGAGAATCCACCTGGGGCTGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8161.01 Da
• Isoelectric Point: 4.0941

>AT108354 WP_015071955.1 NZ_CP031057:541526-541753 [Acidipropionibacterium acidipropionici]
MKLSVSLSKEQVETIDRYIRATGMRSRSAVIQKAIGMLGAPELEGDYAAAWDEWESGDAEAWESTAADGLGDDPR

Nucleotide

• Length: 228 bp

>AT108354 NZ_CP031057:541526-541753 [Acidipropionibacterium acidipropionici]
ATGAAACTCAGTGTCAGCCTGTCCAAGGAGCAGGTGGAGACCATCGACCGGTACATCCGTGCCACCGGGATGAGGTCGAG
GTCCGCGGTGATCCAGAAGGCGATCGGCATGCTCGGCGCCCCGGAGCTCGAGGGGGACTACGCCGCCGCCTGGGACGAGT
GGGAGTCCGGGGACGCCGAGGCCTGGGAGTCGACGGCCGCCGATGGGCTCGGCGATGATCCGCGGTGA

Structures

Toxin

• AlphaFold DB: K7S927

Antitoxin

• AlphaFold DB: K7S105