Detailed information of TA system TA108350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2282847..2283453
• Replicon: chromosome
• Accession: NZ_CP031054
• Organism: Moorella thermoacetica strain 39073-HH

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1D7XDY8
• Locus tag: MothHH_RS11605
• Protein ID: WP_011393652.1
• Coordinates: 2282847..2283197 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MothHH_RS11610
• Protein ID: WP_036371414.1
• Coordinates: 2283199..2283453 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MothHH_RS11565	2278457..2278942	-	486	WP_036371410.1	ribosomal protein S18-alanine N-acetyltransferase	-
MothHH_RS11570	2278960..2279646	-	687	WP_011393646.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
MothHH_RS11575	2279627..2280094	-	468	WP_011393647.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
MothHH_RS11580	2280251..2280826	-	576	WP_011393648.1	uracil-DNA glycosylase	-
MothHH_RS11585	2280881..2281513	-	633	WP_011393649.1	thiamine phosphate synthase	-
MothHH_RS11590	2281594..2281776	-	183	WP_053095107.1	response regulator	-
MothHH_RS11595	2281798..2281983	-	186	WP_011393650.1	response regulator	-
MothHH_RS11600	2282132..2282833	-	702	WP_011393651.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
MothHH_RS11605	2282847..2283197	-	351	WP_011393652.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MothHH_RS11610	2283199..2283453	-	255	WP_036371414.1	ribbon-helix-helix protein, CopG family	Antitoxin
MothHH_RS11615	2283581..2284702	-	1122	WP_025773385.1	alanine racemase	-
MothHH_RS11620	2284674..2286266	-	1593	WP_011393655.1	NAD(P)H-hydrate dehydratase	-
MothHH_RS11625	2286271..2286660	-	390	WP_071556381.1	holo-ACP synthase	-
MothHH_RS11630	2287008..2287997	+	990	WP_011393657.1	AMP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12684.71 Da
• Isoelectric Point: 7.2034

>T108350 WP_011393652.1 NZ_CP031054:c2283197-2282847 [Moorella thermoacetica]
MLVRRGDVFYAHLNPVVGSEQGGTRPVLIIQNDIGNQYSPTTIVAAITSQIAKAKLPTHVEISAAKSGLERDSVILLEQI
RTIDKSRLKQKVAVLDDEILEKVNRALEISLGLVEV

Nucleotide

• Length: 351 bp

>T108350 NZ_CP031054:c2283197-2282847 [Moorella thermoacetica]
ATGCTGGTACGGCGCGGGGATGTCTTTTACGCCCATCTCAATCCGGTGGTCGGTTCCGAGCAAGGCGGAACCCGCCCGGT
ACTTATCATTCAAAATGACATTGGCAACCAGTATAGCCCCACTACTATTGTGGCCGCTATTACTTCCCAGATTGCCAAGG
CCAAACTGCCCACCCATGTGGAAATCAGCGCTGCCAAGAGCGGCCTGGAGCGCGATTCTGTTATTCTGCTGGAACAGATC
CGCACCATAGACAAGAGCCGCCTCAAACAAAAGGTGGCCGTCCTGGATGATGAGATCCTGGAAAAGGTGAACCGGGCCCT
GGAAATAAGCCTGGGACTGGTGGAAGTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9983.45 Da
• Isoelectric Point: 6.3653

>AT108350 WP_036371414.1 NZ_CP031054:c2283453-2283199 [Moorella thermoacetica]
MISLPESLLAEVDGLANLEKRNRSEFIREAMKLYITERKRRNIREQMKRGYQEMAPINLALAVENYDVENEVQRYYDKSL
AECK

Nucleotide

• Length: 255 bp

>AT108350 NZ_CP031054:c2283453-2283199 [Moorella thermoacetica]
ATGATCAGCTTGCCGGAGAGCCTCCTGGCCGAGGTTGACGGCCTGGCGAACCTTGAAAAGCGTAACCGCAGCGAGTTTAT
CCGGGAAGCTATGAAACTATATATTACCGAGCGTAAAAGGCGCAATATCCGGGAACAAATGAAACGGGGATACCAGGAAA
TGGCACCTATCAATTTAGCCCTTGCAGTGGAAAACTATGATGTAGAGAATGAAGTACAAAGATACTACGATAAAAGCCTG
GCGGAGTGTAAATAA

Structures

Toxin

• AlphaFold DB: A0A1D7XDY8